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Biochemistry
Can someone help me calculate protein concentration using the Bradford Assay? If you could show me how to do tube 2, that'd be great. Tube 1: 0 ul Protein Stock Solution, 800 ul Water, 0 ug Protein, Absorbance @595nm = 0 Tube 2:
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I have a hard time with this question. Please help. One way to determine the concentration of protein in a solution is to perform a "protein assay". There are several assay kits on the market that one can use, but they all rely on
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Expressing concentration in units of μg/mL or mg/mL are common in biochemistry, where accurate molecular weights of biomolecules are not always known. The plot below is the standard curve of a Bradford assay, a colorimetric
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If the calibration curve for a Beer's Law plot of a protein assay had a slope of 0.433 when absorb acne, A, was plotted as a function of protein concentration, c, in mg/mL. The absorbance of an unknown protein sample was 0.345.
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If the calibration curve for a Beer's Law plot of a protein assay had a slope of 0.433 when absorb acne, A, was plotted as a function of protein concentration, c, in mg/mL. The absorbance of an unknown protein sample was 0.345.
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