You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the soln. after autoclaving is done. Assume that the diluent is dI water.
You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you
Analysis of Tap water (Hardness) 50.00 mL of tap water was transferred to a 250 mL erlenmyer flask. 10mL of NH3/NH4^+ ph 10 buffer was added to the flask, as well as 6 drops of eriochrome black T indicator. This soln was then
I am trying to calculate the how amount of Tris-bicarbonate should be added in the reaction. I would like to have final volume of bicarbonate 10mM in 2ml reaction. I made Tris-base (1M, 100ml) and instead of HCl, CO2 was bubbling
How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with