In this exercise, you need to spike 0.5 L of river water with 1,000 �g/L of phosphate.
Starting from a stock solution containing 1,000 mg/L of phosphate, calculate and
explain in detail how exactly you will conduct this spiking experiment
To conduct the spiking experiment, you will need to determine the volume of the stock solution required, and then mix it with river water to achieve the desired concentration. Here's how you can calculate and carry out the experiment:
Step 1: Calculate the volume of stock solution needed:
Given that the stock solution contains 1,000 mg/L of phosphate and you want to spike 0.5 L of river water, you can use the formula:
Volume of stock solution (L) = Mass of phosphate needed (g) / Concentration of stock solution (g/L)
In this case, the mass of phosphate needed is 0.5 L x 1,000 g/L = 500 g.
So, the volume of stock solution required will be 500 g / 1,000 g/L = 0.5 L.
Step 2: Obtain the stock solution:
You will need a bottle or container of the stock solution that contains 1,000 mg/L of phosphate. It is important to measure the actual concentration of the stock solution using a calibrated instrument or a reliable method to ensure accuracy.
Step 3: Spike the river water:
To spike the 0.5 L of river water with the correct concentration, follow these steps:
- Measure 0.5 L of river water using a graduated cylinder or any appropriate measuring tool.
- Pour the measured river water into a clean container or vessel.
- Carefully measure and add the calculated 0.5 L of the stock solution (containing 1,000 mg/L) to the container with the river water.
- Mix the contents of the container thoroughly to ensure even distribution of the phosphate throughout the spiked water.
It is crucial to maintain good laboratory practices, such as using clean glassware and preventing contamination, to obtain accurate results. Additionally, ensure proper labeling of the solutions and follow all safety protocols as required for handling the chemicals involved.