The dimensions of each corner square in the haemocytometer are 0.1 cm x 0.1 cm x 0.01 cm. (Hint: 1 cm3 = 1 mL) a) Calculate the volume of a corner square. b) Calculate the density of the cells in your tube (in cells/mL). c) What volume of your cells suspension would you need to pipet if you wanted to seed 1000 cells into 1 WELL of a 6-well plate (well volume = 1 mL)? Provide your answer in microliters (µL). SHOW FULL WORK.
I am not sure how to do c,any tips?
To calculate the volume of the cell suspension needed to seed 1000 cells into one well of a 6-well plate, you need to consider the density of cells in your tube and the volume of each well.
Given:
- Dimensions of each corner square in the haemocytometer: 0.1 cm x 0.1 cm x 0.01 cm
- Well volume: 1 mL
a) Volume of a corner square:
The volume of a rectangular shape can be calculated by multiplying its length, width, and height. In this case, the dimensions are given in centimeters, so we multiply them together to get the volume in cm^3. However, we will also convert the volume to mL since 1 cm^3 is equal to 1 mL.
Volume of a corner square = 0.1 cm x 0.1 cm x 0.01 cm
= 0.0001 cm^3
= 0.0001 mL
b) Density of cells in your tube:
To calculate the density of cells, you need to know the number of cells in the tube and the volume of the tube. Unfortunately, the total number of cells is not provided in the question. To continue, we need this information.
c) Volume of cell suspension needed to seed 1000 cells into 1 well:
Once we have the density of cells in the tube, we can use it to calculate the volume of the cell suspension needed to seed a specific number of cells.
Volume of cell suspension = (Number of cells to seed) / (Density of cells in the tube)
In this case, we want to seed 1000 cells and have the density of cells in the tube (which is missing). Without this information, it is not possible to calculate the volume of the cell suspension needed.
To solve part (c), you will need to know the density of cells in your tube, which is unfortunately not provided in the given information. If you have this information, you can divide the number of cells to seed (1000) by the density of cells in the tube (cells/mL) to determine the required volume of cell suspension.