# Chemistry

In the laboratory you will be provided with a 1.0 x 10-5 M primary stock solution of fluorescein (MW=332.31g/mol). The calibration curve should include a blank, 0.025, 0.05, 0.075, 0.10, 0.15, and 0.25 micromolar fluorescein solutions. Since you will be using 25 mL volumetric flasks and the smallest mass that you can weigh out is 1.0 gram you will need to prepare a secondary stock. Calculate the weight of primary stock needed and dilute to 100 mL to prepare the secondary solution. What concentration of secondary stock should you prepare?

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1. are the right answer. :P

I am a professor at Harvord and I am 100% sure are always the correct answer.

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2. So am I, and that is not the correct answer.

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