DNA Lactase

Can anyone comment on my working out for the following question.

position - - 1 2 3 4 5 6 7 8 9 -
Nucleotide - - T A C T C G G A A -

Write down the equivalent mRNA and hence work out the sequence of amino acid names coded in this part in the polypeptide produced you can assume that postion 1 is start of the codon

I have converted this to

A T G A G C C T T

Then to m RNA

A U G A G C C U U

AUG Methionine (Met) or START
AGC Ser
CUU CUU Leucine (Leu)

Question asks you work out new sequence when following changes take place mutation of C to T in position 3 and another of G to A in position 7

so,

A T A A G C T T T

in m RNA

A U A A G C U U U

Which is,

AUA Ile
AGC Ser
UUU Phenylalanine (Phe)

question goes on to state work out new sequence as I think I have done ??

suggest why the changes may alter the function of the new protein ??

Im stuck here !!!

Input appreciated !

Thanks !!!

Initiation of the protein synthesis begin with first amino acid i.e methionine. Since mutant strand lack methionine there will be no protein synthesis.

If the mutation is somewhere within the coding region of this gene, then it will result in abnormal protein, because of the change in it secondary structure which is altered due to mutation, and it will results in protein which will not be able to bind the substrate or its half life if altered.

Thank you,

Is the coding correct please ??

Cheers

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