when subcloning a gene and inserting it into a vector you normally would use x-gal to locate transformants if x-gal is not available what are the alternatives

<<In addition to X-Gal, several alternative [3-gal sub-
strates with different halide groups on the indolyl rings are commercially available. These can be substituted directly for X-Gal in the staining procedures described here, each generating a characteristic color
and resolution of staining [ 1 l] (Fire and Xu, unpublished). Two derivatives, 5-bromo-3-indolyl-13-D-galactoside ("bluo-gal" [BRL]) and 6-chloro-3-in-
dolyl-13-o-galactoside ("Salmon-gal" [Biosynth AG] or "Red-Gal" [Research Organics]), give more dif
fuse staining than X-Gal. A more useful derivative for detailed histochemistry is 5-bromo-6-chloro-3-indolyl-13-D-galactoside ("Magenta-gal" [Biosynth
AG]), which yields magenta staining with definition comparable to X-Gal. The latter substrate should be
particularly valuable in applications involving localization of two different enzymes (for example, 13-gal
and alkaline phosphatase). >>

  1. 👍
  2. 👎
  3. 👁

Respond to this Question

First Name

Your Response

Similar Questions

  1. Calculus and vectors

    Vector AB is a vector whose tail is at (-4,2) and whose head is at (-1,3). Calculate the magnitude of vector AB Determine the coordinates of point D on vector CD, if C (-6,0) and vector CD= vector AB. Please I need some help. Is

  2. Physics

    You are given vectors A = 5.0i - 6.5j & B = -3.5i + 7.0j. A third vector C lies on the xy-plane. Vector C is perpendicular to vector A, & the scalar product of C with B is 15.0. From this information, find the components of vector

  3. Science

    Which statement best describes a dominant gene? A. It Is the Gene that produces mutations. B. It Is a gene that produces desirable traits.** C. It Is the Gene that masks a recessive Gene. D. It Is a gene that Is masked by a

  4. Math

    Suppose that 30% of a population carries a certain gene. You want to estimate the probability that it will take at least a sample of four to find a person that carries the gene, how could you simulate a person having or not having

  1. Vectors

    Explain why it is not possible for Vector a • (Vector b • Vector c) to equal (Vector a • Vector b) • Vector c . (This means that the dot product is not associative.)

  2. Vectors

    Verify using an example that Vector a + (Vector b • Vector c) is not equal to (Vector a + Vector b) • (Vector a +Vector c). (This means that addition does not distribute over the dot product.) Explain the problem that arises.

  3. Dot Product

    Verify using an example that Vector a • (Vector b • Vector c) = (Vector a • Vector b) • Vector c is not true. Explain your reasoning both numerically and by using the definition of the dot product. I am very confused as to

  4. calculus

    An automobile computer gives a digital readout of fuel consumption in gallons per hous. During a trip, a passenger recorded the fuel consumption every 5 minutes for a full hour of travel. time: 0 / gal/h: 2.5 time: 5 / gal/h: 2.4

  1. math vectors

    Use a specific example to prove that the cross product is also not associative. That is, use three specific vectors in 3-space to show that Vector a×(Vector b × Vector c) is not equal to (Vector a × Vector b) × Vector c. Can

  2. chemistry

    A particular brand of gasoline has a density of 0.737 g/mL at 25 ∘C. How many grams of this gasoline would fill a 13.2 gal tank (1US gal=3.78L)?

  3. Physics

    A farmer builds three sections of a fence as shown in the figure where L = 10.00 m, and gets tired. Walking straight back to where he started, how far did he go. d=? Vector on Y-axis going to origin, L/2 vector going on Positive X

  4. calculus

    State whether or not the following statements are true. Justify your reasoning.? a. Vector a • (Vector b + Vector c) = Vector a • Vector b + Vector a • Vector c b. Vector a × (Vector b + Vector c) = Vector a × Vector b +

You can view more similar questions or ask a new question.