How can I tell which of two mobile phases is more polar? Does the fact that one solvent (e.g. isopropanol/ammonia) moves up the TLC plate slower than another solvent (e.g. hexane/ethyl acetate/ethanol)?
Or should I look at how far up the dyes have moved?
I think you can use both methods. The first one if you compare the move up the plate IF you know that the TLC plate is coated with a polar/non-polar material. The second IF you know the polarity of the dyes.
Chromatography How can I tell which of two mobile phases is more polar? Does the fact that one solvent (e.g. isopropanol/ammonia) moves up the TLC plate slower than another solvent (e.g. hexane/ethyl acetate/ethanol)? Or should I
If i have a highly polar mobile phase, what will happen to the non-polar or less polar compounds during chromatography? will they move less up the TLC plate or actually even more because the mobile phase itself is so polar.
Chromatography relies on the differences in polarity between the compound, the mobile phase and the stationary phase. In your own words explain how polar and non-polar compounds can be separated using cellulose paper and petroleum
I have a question on thin layer chromatography (TLC). My mobile phase (i.e. developing solvent) is a mixture of 2 non-polar solvents. After preparing the mobile phase, is it necessary to pour some into the developing chamber to
What type of substance is the mobile phase in gas chromatography? a polar liquid a nonpolar liquid a mixture of two liquids to create the correct polarity a polar gas an inert gas like nitrogen my answer is an insert gas like
For a TLC lab,the stationary phase is the mixture of isopropanol and ammonia or the mixture of hexane,ethyl acetate and ethanol, the stationary phase is silica gel. text some dyes and drugs. which one of the two mobile phases is
What is the mobile phase in this experiment? A) 10% 6M NaOH and 90% acetone B) 10% 6M HCl and 90% acetone C) 10% 5M KCl and 90% methanol D) 10% 5M KCl and 90% acetone Gel electrophoresis is an example of which type of
We did a column chromatography then a TLC on extracted spinach pigments. Question: Why are the chlorophylls less mobile on column chromatography and why do they have lower Rf values than the carotenes? My answer: The chlorophylls
Is the the rf value of lycopene and with what solvent can you use as a mobile phase on a TLC? I don't know the answer here but I looked on the Internet and found something that may get you started. I found the Rf value for
My mobile phase for HPLC (High Performance Liquid Chromatography) test consists of 3 components in the ratio 5:250:745. In preparing the mobile phase, is it advisable to first measure 745ml of component A, followed by 250ml of