how do I calculate rate velocity using
absorbence at 412/ min-1.
To calculate the rate velocity using absorbance at 412 nm per minute (min⁻¹), you need to follow these steps:
1. Determine the change in absorbance (ΔA):
- Collect absorbance measurements at two different time points.
- Subtract the initial absorbance value (A₁) from the final absorbance value (A₂). This gives you the change in absorbance (ΔA = A₂ - A₁).
2. Determine the change in time (Δt):
- Note the time difference between the two absorbance measurements. This is the change in time (Δt = time₂ - time₁).
3. Calculate the rate of change in absorbance per minute:
- Divide the change in absorbance (ΔA) by the change in time (Δt). This will give you the rate of change in absorbance per unit time (rate velocity).
- Rate Velocity = ΔA / Δt
For example, if you have measurements of absorbance A₁ = 0.2 at time t₁ = 0 minutes and A₂ = 0.8 at time t₂ = 5 minutes, you can calculate the rate velocity as follows:
ΔA = A₂ - A₁ = 0.8 - 0.2 = 0.6
Δt = t₂ - t₁ = 5 - 0 = 5 minutes
Rate Velocity = ΔA / Δt = 0.6 / 5 = 0.12 min⁻¹
Therefore, the rate velocity is 0.12 min⁻¹ based on the given absorbance measurements.