Biochemistry

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You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1.
What is the concentration of the components (Tris and Tris-H+) in the
final buffer solution?

  • Biochemistry -

    Use the Henderson-Hasselbalch equation. That is equation 1.
    7.5 = 8.1 + log (tris)/(tris-HCl)

    equation 2 is:
    tris + tris-HCl = 50 mM

    Solve the two equations simultaneously to determine tris and trish-H^+

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