Chemistry

posted by .

How do you create a calibration curve? How do you use a calibration curve to convert absorbance to concentration in mg/mL?

  • Chemistry -

    You make a set of standards (solution of known concentration of the molecule/ions you want to determine), run them through the same procedure you will use on the unknowns, then measure the absorbance of each. Plot A on the y axis and mg/mL on the x axis. That is your calibration curve. Then you run the unknowns, measure A values, use the calibration curve to convert A to mg/mL and report your results.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Chemistry

    I'm trying to do a lab, and I need to find the concentration of FeSCN+2 in five solutions based the calibration curve and the absorption. I have a calibration curve, the number of mLs and initial moles of Fe+3 and SCN-, the transmittances, …
  2. chemistry-calibration curve

    what does the calibration curve tell you about the spectrophotometer?
  3. chem

    Joe Student created a calibration curve. When determining the unknown concentration of the same chemical, he found the absorbance to be 0.02. What is the unknown concentration?
  4. Chemistry (URGENT)

    In order to analyze the waste water containing Co^2+ from a manufacturing process, 1.0L of water was evaporated to 10.0mL, then placed in a spectrophotometer tube. The absorbance was found to be 0.20. Using your calibration curve, …
  5. Chemistry

    Refer to the calibration curve shown in the experiment. If the absorbance of the solution is 0.400, what is the concentration of the iron in that solution?
  6. Chemistry

    Do I solve this by multiplying 1.8645 by 1.14?
  7. Chem

    If the calibration curve for a Beer's Law plot of a protein assay had a slope of 0.433 when absorb acne, A, was plotted as a function of protein concentration, c, in mg/mL. The absorbance of an unknown protein sample was 0.345. What …
  8. Biochem

    If the calibration curve for a Beer's Law plot of a protein assay had a slope of 0.433 when absorb acne, A, was plotted as a function of protein concentration, c, in mg/mL. The absorbance of an unknown protein sample was 0.345. What …
  9. Science

    Propose a series of concentration to be used for calibration curve. If an unknown solution produced a result outside the range of the calibration curve, how could this be solved?
  10. Chem

    Calibration data for a bromide ion-selective electrode (ISE) was collected and recorded below. The potential of the ISE was measured against a saturated calomel electrode (SCE). All solutions were buffered at a pH of 7.56. A linear …

More Similar Questions