biology

posted by .

A common first step in the making of a DNA library is to obtain DNA from many of your chosen organisms and cut this source DNA with a restriction enzyme. It is likely that some genes will contain sites for the chosen restriction enzyme, and in this case, your DNA library will not have a complete set of genes from your chosen source. There are several ways to avoid this problem, but one solution is to add smaller amounts of the restriction enzyme or decrease the time that the DNA is exposed to active enzyme. In the box below, explain why this approach may allow you to construct a more complete library.

unanswered

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. dna strands/sequences

    The restriction enzyme EcoR1 recognizes the DNA sequence GAATTC. Which DNA strands will be cut by EcoR1?
  2. biology

    How is recombinant DNA technology used today?
  3. bio

    I did a DNA fingerprinting lab and I can't understand some of the questions. 1) Different restriction enzymes are isolated from different types of bacteria. What advantage do you think bacteria gain by having restriction enzymes?
  4. psychology

    Which one of the following statements describe the relationship between genes,chormosomes and DNA?
  5. psychology

    Which one of the following statements describe the relationship between genes,chormosomes and DNA?
  6. Genes

    Which one of the following statements describes the relationship between genes, chromosomes and DNA a) genes, the basic unit of heredity, are located on chromosomes, which consist of strands of DNA b) genes are composed of chromosome …
  7. Biology

    The polymerase chain reaction (PCR) is useful for: A) making many copies of a small amount of DNA B) creating recombinant plasmids C) analyzing a person’s fingerprints D) cutting DNA into many small pieces E) allowing restriction …
  8. psychology

    Which one of the following statements describes the relationship between genes, chromosomes, and DNA?
  9. Psychology

    Which of the following statements describes the relationship between genes, chromosomes, and DNA?
  10. Biology (cell-molec)

    You wish to perform a restriction digest in a 20uL volume. Your DNA is 0.20 ug/uL and you wish to digest 1.0 ug. Your restriction enzyme is provided as 0.2 U/uL. Fill in the appropriate volumes: H2O, 10Xbuffer, DNA, and restriction …

More Similar Questions