posted by BaileyBubble .
Suppose you are a scientist trying to help people who cannot produce an enzyme needed for proper digestion. How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
somebody please help me :'(
Since this is not my area of expertise, I searched Google under the key words "genetic transformation of enzyme producing bacteria" to get these possible sources:
In the future, you can find the information you desire more quickly, if you use appropriate key words to do your own search. Also see http://hanlib.sou.edu/searchtools/.
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.