Chemistry-please help

posted by .

Hi,
I have a queestion on Gas Chromatography(GC).
The following were injected into the GC systemk: -

1) 1% sample solution in acetone
2) a 500 times dilution of item 1)

The retention time of the main peak in the diluted sample solution 2) shifted by ~ 2min.
The expected area count is also half of what is theoretically calculated. (i.e. I would expect the area count of the diluted solution to be 500 times less than the sample solution.)

Can you suggest possible reasons for this difference in retention time between the sample and the diluted sample?
Does this shift in retention time happen to some samples that are too diluted?

Thank you.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Chemistry

    In gas chromatography, what would be the order of the retention times for your mixture (hexane and toluene)?
  2. Chemistry (lab)

    What are the units of retention time in gas liquid chromatography?
  3. Organic Chemistry Lab

    A sample of hexane and octane is injected into a gas chromatograph equipped with a nonpolar column. Predict which one will have the shorter retention time.
  4. chemistry

    a sample of hexane and octane is injected into a gas chromatograph equipped with a non-polar column. predict which will have the shorter retention time
  5. Chemistry-help

    Hi, I have a question on Gas Chromatography(GC). The following were injected into the GC system: - 1) 1% sample solution in acetone 2) a 500 times dilution of item 1) The retention time of the main peak in the diluted sample solution …
  6. Chemistry-Bob please help

    Posted by anne on Friday, October 14, 2011 at 6:30pm. Hi, I have a question on HPLC (high performance liquid chromatography). The procedure says to use a 4.6 x 150mm x 3um column. The relative retention time of Impurity B with reference …
  7. Chemistry

    In a chromatography experiment, a solution was prepared by mixing 12.98 mg of isopropanol plus 10.00 mL of an unknown containing some acetone, and diluting to 25.00 mL. Signal of 5.97 and 6.38 were observed for the acetone and isopropanol, …
  8. Chemistry

    If the gas chromatograph is polar, and a mixture of a polar compound and a non-polar compound is injected. Which one of the two compounds will have a higher retention time?
  9. science - gas chromatography

    why is retention and peak width measured in time?
  10. Chemistry

    What is the mobile phase in this experiment?

More Similar Questions