posted by Sue

You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the soln. after autoclaving is done. Assume that the diluent is dI water.

Respond to this Question

First Name

Your Answer

Similar Questions

  1. Biology

    The following chemicals have the indicated formula weight Surcose = 342.3g; Potassium dihydrogen=136.09; and Tris=121.14 Also the following solution already made: 20%SDS, 5M NaCl, 500mM EDTA. You have a 40% glycerol solution, and 10X …
  2. Biochem

    Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)
  3. Biology

    For one of our lab sessions, we had to isolate DNA from E. Coli bacteria. During one of the steps in the isolation process, we had to use SDS(Sodium lauryl sulfate) at 60°C. One of the questions was: Why do we use SDS, and why at …
  4. Chemistry 3A

    You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will …
  5. chemistry

    Sodium dodecyl sulfate (SDS) has the structure below. The surface tension of water changes as SDS is added, as shown in the graph below. (a) Why does the surface tension of water change as SDS is added?
  6. chemistry

    How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM?
  7. Molecular cell Biology

    The following chemicals have the indicated formula weights:Sucrose-342.3,Potassium dihydrogen phosphate(KH2PO40-136.09,Tris -121.14.You also have the following solutions made:20%SDS,5M NaCl,500mM EDTA.You have a 40%glycerol solution …
  8. biochemistry

    how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175
  9. Biochemistry

    You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?

    1× SDS Running Buffer For this exercise, you will need to prepare 1 L of 1x SDS Running Buffer. The final composition of this buffer is: 25 mM Tris, 200 mM Glycine pH 8.3, 0.1% (w/v) SDS 1. To a 1 L beaker, add 500 ml of distilled …

More Similar Questions