engineering

posted by .

when i do imaging of tissue to look at collagen structure using a 2 photon microscopy, but im not familiarized with the methods to measure the diameter of the collagen and the area occupied by it. Does anyone know how do researchers measure them

  • bioengineering & microscopy -

    I am not familiar with the technique and had to look it up here:
    http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy

    The device has a scanning mirror. It should be precalibrated for spatial scale. Check the instruction manual for the spatial field of view at various magnification settings

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Physics

    I think that for energy of a spring I should use (1/2)(k)(x^2) and (1/2)(m)(v^2) but i'm not sure what to do with the equations to solve the problem. A cylindrically shaped piece of collagen is being stretched by a force that increases …
  2. engineering

    does anyone know how can I measure collagen's diameter in tissue using what kind of a microscope
  3. biology

    I want to imaging collagen and elastin in porcine heart tissue without staining using a confocal microscopy. So far most of the articles that I found are using staining to differentiate the two, is anyone familiar with the microscopy …
  4. Physics

    A cylindrically shaped piece of collagen is being stretched by a force that increases from 0 to 3.0 x 10^-2 N. The length and radius of the collagen are 2.5 and 0.091 cm. Young's Modulus is 3.1 x 10^6 N/m^2. a.) If the stretching obeys …
  5. College Physics PLEASE HELP

    A cylindrically shaped piece of collagen is being stretched by a force that increases from 0 to 3.0 x 10^-2 N. The length and radius of the collagen are 2.5 and 0.091 cm. Young's Modulus is 3.1 x 10^6 N/m^2. a.) If the stretching obeys …
  6. Anatomy Physiology 1

    1) How would the effects of a collagen vascular disease that affected only the collagen in reticular fibers differ from the effects of collagen fibers on organs & tissues?
  7. Mechanical Behavior of Materials

    Calculate the load that a fibroblast cell applies to buckle a single column in a collagen tissue engineering scaffold, given that the column has a length of 100μm, is circular in cross section, with a diameter of 2μm, and …
  8. broward college

    collagen vascular disease ,affect the protein collagen ,the main component of collagen fibers. predict the possible consequence of defective collagen fibers on specific organs and tissues how would effect of collagen vascular disease …
  9. bio 230

    how would the effects of a collagen vascular disease that affected only the collagen in reticular fibers
  10. Physics

    A cylindrically shaped piece of collagen (a substance found in the body in connective tissue) is being stretched by a force that increases from 0 to 0.0436 N. The length and radius of the collagen are, respectively, 2.90 cm and 0.0815 …

More Similar Questions