Biochemistry

posted by .

Assuming pKa values of 3.8 for the a-COOH and 8.5 for the a-amino groups and that the numbers in parentheses indicate the pKa values of side chain groups, at what pH would electrophoresis provide the best resolution of the following dipeptides?

His (6.0) – Ser

Gly – Lys (10.0)

I think I'm missing the theory behind this. If the side chain and the pH are both acidic, does that mean it's better?

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Biochemistry

    Find the net charge of cysteine at pH 8.75 using the follwing pka values: carboxyl group has pka=1.9 amino group pka= 10.7 side chain=8.4
  2. biochem

    How do you draw a tetrapeptide from the one letter codes(Amino acids) and also give the pI, if given the pKa values?
  3. bio

    How do you draw a tetrapeptide from the one letter codes(Amino acids) and also give the pI, if given the pKa values?
  4. chemistry buffers

    What molar ratio of HPO4 2- to H2PO4 - in solution would produce a pH of 7.0?
  5. Analytical Chemistry

    Show the predominant form of the amino acid tyrosine at pH=13.9 and ph=7.8. Be sure to include the proper number of hydrogens on heteroatoms and the correct formal charges on atoms. I calculated the pka values for COOH (pka=2.41) NH3 …
  6. Chemistry

    Calculate the pI (isoelectric point) of the dipeptide, Lys-Gly, assuming that there is negligible shift in the values of acid dissociation constants from the values of the constituent amino acids. Gly pkA 9.87 an pka 2.35; Lys pka …
  7. chemistry

    The skeletal structures of the two amino acids, glycine and lysine, are given below along with the values of the relevant acid dissociation constants (pKa). (b) Calculate the pI (isoelectric point) of the dipeptide, Lys-Gly, assuming …
  8. CHEMISTRY !!! HELP !

    A 70.0 mL solution of 0.165 M potassium alaninate (H2NC2H5CO2K) is titrated with 0.165 M HCl. The pKa values for the amino acid alanine are 2.344 (pKa1) and 9.868 (pKa2), which correspond to the carboxylic acid and amino groups, respectively. …
  9. CHEMISTRY

    A 70.0 mL solution of 0.165 M potassium alaninate (H2NC2H5CO2K) is titrated with 0.165 M HCl. The pKa values for the amino acid alanine are 2.344 (pKa1) and 9.868 (pKa2), which correspond to the carboxylic acid and amino groups, respectively. …
  10. Biochemistry

    All amino acids have two ionizable groups (an alpha-amino group with pKa~9.3, and an alpha-carboxyl group with pKa~2.2). Lysine also has an ionizable side-chain (R) with a pKa of 10.5. What percentage of the Lysine side chain is deprotonated …

More Similar Questions