chemistry please help

posted by .

An enzyme sample contains 24 mg protein/mL. Of this sample, 20
microliters in a standard incubation volume of 0.1 mL catalyzed the
incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the
velocity of the reaction in terms of the following:

A)micromoles/minute
B)micromoles/liter/minute
C)micromoles/miligrams of protein/minute
D)Units/milliliter
E)Units/miligrams of protein


Of this sample, 50 mL were fractionated by ammonium sulfate
precipitation. The fraction precipitating between 30 and 50% saturation was
redissolved in a total volume of 10 mL and dialyzed. The solution after
dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction,
20 microliters catalyzed the reaction rate of 5.9 nmol/min under the
standard assay conditions. calculate the following:

A. The recovery of enzyme after the ammonium sulfate step.
B. The fold purification after the ammonium sulfate step.

note: for recovery: divide total units of he sample by the total units of the crude homogenate and multiply by 100.
for fold purification: it is the ratio of the specific activity of a given fraction divided by the specific activity of the crude homogenate.

  • chemistry please help -

    Note: a Unit is ((ΔA/Δmin)/(6220 M -1 cm -1)) X 106 μM/M X 3 X 10-3 L

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Biochemistry

    Consider the following kinetic parameter for a given enzyme: Km=4.7x10-5 M Vmax= 22 nmol/min/mg [i]=5x10-1 mM [s]=2x10-4 M Ki=3x10-4 M Calculate the rate of product formation in the presence of a competitive inhibitor. I know that …
  2. chemistry

    Consider the following kinetic parameter for a given enzyme: Km=4.7x10-5 M Vmax= 22 nmol/min/mg [i]=5x10-1 mM [s]=2x10-4 M Ki=3x10-4 M Calculate the rate of product formation in the presence of a competitive inhibitor. I know that …
  3. chemistry

    Assayed for LDH activity were 5 microliters of a sample that was diluted 6 to 1. The activity in the reaction vessel, which has a volume of 3 mililiters, is 0.30 U. What is the ΔA/min observed?
  4. chemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated …
  5. biochemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the velocity of the reaction …
  6. chemistry

    A 1.10g- sample contains only glucose and sucrose . When the sample is dissolved in water to a total solution volume of 25.0ML , the osmotic pressure of the solution is 3.78atm at 298K a.What is the percent by mass of glucose in the …
  7. Chemistry

    A 25.00 ml sample of a standard solution containing 1 g of CaCO3/L required 25.20 ml of EDTA to fully complex the Ca present. Calculate the volume of EDTA stoichiometrically equivalent to 1.0 mg of CaCO3. From the volume of EDTA required …
  8. Chemistry

    consider 2 sample of nitrogen gas. sample 1 contains 1.5 mol of N2 and has a volume of 36.7L at 25C and 1 atm. sample 2 has a volume of 16.5L at 25C and 1 atm. calculate the number of moles of N2 in sample 2 do i use the pv= nrt equation?
  9. Biology

    Hi, I am doing my lab assignment about Protein Analysis. Our job is to find the concentration of the casein protein in our original Unknown sample with the initial volume of 2ml. So after plotting the graph of the data given, we are …
  10. Chemistry

    An enzyme has velocities of 0.11 nmol/min at a substrate concentration of 1 μM, 0.34 nmol/min at a substrate concentration of 5 μM, and 0.45 nmol/min at a substrate concentration of 10 μM. What is the Vmax for the enzyme …

More Similar Questions