Dissolve 0.1gm of cane sugar in 2cc if water. To this dilute solution in a test tube, add 2 drops of molischs reagent and shake. Carefully pour 2cc o conc H2SO4 down the side of the tube and allow to stand for 2 minutes
By following the given steps, you have created a test to detect the presence of carbohydrates, such as cane sugar, using Molisch's reagent. The steps are as follows:
1. Measure 0.1 grams of cane sugar.
2. Add the measured cane sugar into a test tube.
3. Measure 2 cc (cubic centimeters) of water and pour it into the test tube with the cane sugar.
4. Shake the test tube to dissolve the cane sugar in the water.
5. Add 2 drops of Molisch's reagent to the test tube.
6. Shake the test tube again to mix the reagent with the solution.
7. Take 2 cc of concentrated sulfuric acid (H2SO4) and carefully pour it down the side of the test tube. Be cautious while handling the concentrated acid.
8. Allow the test tube to stand undisturbed for 2 minutes.
After following these steps, you would observe the formation of a purple ring at the junction of the two liquids if a carbohydrate is present in the solution.