Molischs test
Dissolve 0.1gm each of glucose, cane sugar and dextrin in 2cc of water. To this dilute solution in a test tube, add 2 drops of molischs reagent and shake. Carefully pour 2cc of conc H2SO4 down the side o_ hre rube and allow to stand for 2 minutes. State your observations
Molisch's test is used to detect the presence of carbohydrates in a given solution.
Observations:
- After adding 2 drops of Molisch's reagent to the test tube containing the solution, a deep purple coloration will be observed.
- When 2 cc of concentrated H2SO4 is carefully poured down the side of the tube, it will form a layer at the bottom of the tube.
- The tube is allowed to stand for 2 minutes.
- As a result of the reaction between carbohydrates and the reagents, a brown ring will form at the junction of the two layers (purple solution and concentrated H2SO4 layer) indicating the presence of carbohydrates in the solution.
Please note that these observations are based on the assumption that the glucose, cane sugar, and dextrin samples do contain carbohydrates.