Which reason best explains why dead specimens must be used with transmission electron microscopes?
Electrons pass over the specimen.
The lights that are used are harmful to the specimens.
Specimens are placed in a vacuum.
The image that is produced is two dimensional.
The correct reason is: The image that is produced is two dimensional.
Transmission electron microscopes are used to study the internal structure of specimens at a high magnification and resolution. The image produced by this type of microscope is a 2D shadow-like image, and dead specimens are typically used because living specimens would move or be damaged during the intense electron beam irradiation required by the microscope. Additionally, the vacuum environment in transmission electron microscopes is not suitable for living organisms.