Which reason best explains why dead specimens must be used with transmission electron microscopes?

Electrons pass over the specimen.
The lights that are used are harmful to the specimens.
Specimens are placed in a vacuum.
The image that is produced is two dimensional.

The reason that best explains why dead specimens must be used with transmission electron microscopes is that electrons pass over the specimen.

The reason why dead specimens must be used with transmission electron microscopes is because specimens are placed in a vacuum.