In mice a single gene determines the color of the eyes. The black eyes allele is dominant.it is indicated by “E”. The red allele is recessive, and indicated by “e” using a punnet square cross a heterozygous male (Ee) with a heterozygous female (Ee).

To set up a Punnett square for this cross, we would first list the genotypes of the two parents:

Male (Ee)
Female (Ee)

Next, we would create a 4x4 Punnett square to show all possible combinations of alleles that could result from this cross:

| E | e |
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E | EE | Ee |
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e | Ee | ee |
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From this Punnett square, we can see that there is a 25% chance of the offspring having the genotype EE (black eyes), a 50% chance of the offspring having the genotype Ee (black eyes), and a 25% chance of the offspring having the genotype ee (red eyes).