Which conflict caused Spain to raise an armada in the 1500s?

English trade in the New World
the French takeover of land in North America
Europeans’ enslavement of people in Africa
Portuguese claims of gold in South America

The conflict that caused Spain to raise an armada in the 1500s was the English trade in the New World. This led to the Anglo-Spanish War, in which Spain attempted to assert its dominance over the lucrative trade routes and colonies in the New World.