A particular tRNA is mutated so that the amino acid attachment cannot bind with the animoacyl-tRNA synthase. What happens when an mRNA transcript contains the codon for this tRNA?

The tRNA will not bind to this codon.

Translation stops and the protein is released.

The wrong tRNA is added to the protein chain.

Translation stops and the protein remains bound to the ribosome.

Translation stops and the protein remains bound to the ribosome.