Directions:
Arrange the numbered items in the proper order that tells the best story.
1. Phenotypes resulting from siRNA treatment of larval proto-eyes. A) Typical phenotype
resulting from embryo injection with scrambled siRNAs. B) Phenotype resulting from injection of ney
siRNA. C) Phenotype resulting from injection of siRNA specific to novel gene JY.8675309.
2. Phenotypes of mutants recovered from F3 mutagenic
screen. (A) Mutagenesis and genetic screen strategy. (B) wild type (C) no
eye mutant (D) little eye 1 mutant (E) little eye 2 mutant (F) ley1 ley2 double
mutant (G) ney ley1 double mutant (H) ney ley2 double mutant.
3. Correlations between mutant and wild type larval transcript levels. In each
panel a dot is color-coded probes to match the gene on x or y axis. The blue arrow
indicates an unknown transcript (JY.8675309).
DATASET B
4. Predicted domains of proteins encoded by newly identified genes.
Novel = no known function, TM = putative transmembrane domain, PB = putative protein binding
domain, kinase = putative kinase activity.
5. Subcellular localization of JY.867 in cultured cell line. A) Background fluorescence in
untransfected cells. B) Cells transfected with expression plasmid encoding JY.8675309. C) Cells
cotransfected with Ley2 and JY.8675309 expression plasmids. D) Cells cotransfected with
expression plasmids encoding Ley2, JY.8675309, and short hairpin specific to Ley1 mRNA.
The proper order that tells the best story is:
4. Predicted domains of proteins encoded by newly identified genes.
Novel = no known function, TM = putative transmembrane domain, PB = putative protein binding domain, kinase = putative kinase activity.
2. Phenotypes of mutants recovered from F3 mutagenic screen. (A) Mutagenesis and genetic screen strategy. (B) wild type (C) no eye mutant (D) little eye 1 mutant (E) little eye 2 mutant (F) ley1 ley2 double mutant (G) ney ley1 double mutant (H) ney ley2 double mutant.
3. Correlations between mutant and wild type larval transcript levels. In each panel a dot is color-coded probes to match the gene on x or y axis. The blue arrow indicates an unknown transcript (JY.8675309).
5. Subcellular localization of JY.867 in cultured cell line. A) Background fluorescence in untransfected cells. B) Cells transfected with expression plasmid encoding JY.8675309. C) Cells cotransfected with Ley2 and JY.8675309 expression plasmids. D) Cells cotransfected with expression plasmids encoding Ley2, JY.8675309, and short hairpin specific to Ley1 mRNA.
1. Phenotypes resulting from siRNA treatment of larval proto-eyes. A) Typical phenotype resulting from embryo injection with scrambled siRNAs. B) Phenotype resulting from injection of ney siRNA. C) Phenotype resulting from injection of siRNA specific to novel gene JY.8675309.