Which evidence best supports the claim that mutations are a source of inheritable genetic variation?

Mutations affect the way DNA is created through transcription.
Mutations can only happen during meiosis because genes are inherited
Mutations affect protein synthesis which impacts the way traits are expressed
Mutations are not inheritable from gametes because they are given from each parent

The evidence that best supports the claim that mutations are a source of inheritable genetic variation is that mutations affect protein synthesis which impacts the way traits are expressed.