What causes a gray crescent to appear in many frog embryos?

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a
The gray crescent appears as an indentation representing the start of the first cleavage.

b
The outer (cortical) cytoplasm rotates relative to the internal cytoplasm.

c
A thickening of the cytoplasm occurs at the site of sperm entry.

d
The sperm centrioles are gray and provide color to the cytoplasm.

a

The gray crescent appears as an indentation representing the start of the first cleavage.