My student is performing a Benedict's test on an unknown substance the student adds the reagent Chemical required to make the color change if nothing happens which conclusion can the student make

If nothing happens after adding the reagent required for the Benedict's test, the student can conclude that the unknown substance does not contain reducing sugars. Benedict's reagent is specifically used to test for the presence of reducing sugars, such as glucose and fructose. If there is no color change, it means that the unknown substance does not contain any of these sugars.