You take 0.03 ml of the flowthrough fraction, measure the absorbable using the same conditions as in the standard curve above, and get a reading of 0.52. What is the protein concentration of the flowthrough fraction (mg/ml)? y=12.235x
To determine the protein concentration of the flowthrough fraction, we need to use the equation:
y = 12.235x
where y is the absorbance reading and x is the protein concentration (mg/ml).
Given that the absorbance reading is 0.52 and the equation is y = 12.235x, we can rearrange the equation to solve for x:
0.52 = 12.235x
Divide both sides of the equation by 12.235:
0.52/12.235 = x
x = 0.0425
Therefore, the protein concentration of the flowthrough fraction is 0.0425 mg/ml.
To find the protein concentration of the flowthrough fraction, you can use the equation provided, which is y = 12.235x. In this equation, y represents the absorbance reading and x represents the protein concentration.
Given that you have a reading of 0.52 for the absorbance (y), you need to solve for x (the protein concentration). Rearranging the equation, we have:
x = y / 12.235
Substituting the value of y (0.52) into the equation, we get:
x = 0.52 / 12.235
Using a calculator, divide 0.52 by 12.235:
x ≈ 0.0425
The protein concentration of the flowthrough fraction is approximately 0.0425 mg/ml.
To calculate the protein concentration of the flowthrough fraction, we need to use the equation provided: y = 12.235x.
First, let's rearrange the equation to solve for x (protein concentration in mg/ml):
x = y / 12.235
Given that the reading (y) is 0.52, we can substitute it into the equation:
x = 0.52 / 12.235
Using a calculator, divide 0.52 by 12.235:
x ≈ 0.0425
Therefore, the protein concentration of the flowthrough fraction is approximately 0.0425 mg/ml.