The molecule that provides the activation energy required to start glycolysis is ATP (adenosine triphosphate). ATP is a high-energy molecule that acts as the main source of energy for cellular processes. It is involved in transferring energy within cells by providing phosphate groups to other molecules, which can activate or energize them. In the case of glycolysis, ATP donates a phosphate group to glucose, which helps in breaking down glucose and initiating the glycolytic pathway. So, the correct answer is ATP.