Answer these questions in complete sentences:
a. When should the coarse adjustment knob be used?
b. Why should you NOT use the coarse adjustment knob on medium and high power?
c. How do you find total magnification on the microscope?
d. What is the difference between the compound light microscope and stereomicroscope?
e. What is the proper way to hold/move a microscope?
a. The coarse adjustment knob should be used when initially focusing on a specimen under low power.
b. The coarse adjustment knob should not be used on medium and high power because it might result in crashing the objective lens into the slide or specimen, causing damage.
c. Total magnification on a microscope can be found by multiplying the magnification of the objective lens by the magnification of the eyepiece lens.
d. The compound light microscope is used for viewing small, transparent specimens and uses a beam of light passing through the specimen, while the stereomicroscope is used for viewing larger, solid objects and provides a three-dimensional view.
e. The proper way to hold and move a microscope is to grasp it firmly by the arm and base, keeping it upright and close to the body while walking.
a. The coarse adjustment knob should be used to initially bring the specimen into rough focus when using low power objective lens.
b. The coarse adjustment knob should not be used on medium and high power because it could potentially damage the slide or the objective lens, as it moves the stage too quickly and abruptly.
c. To find the total magnification on a microscope, multiply the magnification of the objective lens being used by the magnification of the eyepiece lens.
d. The compound light microscope is used for viewing highly magnified, thin specimens, such as cells or microorganisms, and it uses transmitted light. The stereomicroscope (also known as a dissecting microscope) is used to view larger, solid specimens in three-dimensional form, and it uses incident light.
e. The proper way to hold and move a microscope is to place one hand under the base and the other hand on the arm of the microscope. This provides stability and control while moving the microscope around. Avoid gripping the microscope by the stage or the coarse/fine adjustment knobs.