The following piece of double stranded DNA encodes a very
short peptide. In the space below, write the sequence of
mRNA that would be synthesized by RNA polymerase from
this piece of DNA if the bottom strand is the template. Then
write the protein sequence in one-letter code that would be
synthesized by the ribosome from that mRNA using the first
reading frame (use Genetic Code)
i. 5’-ATGGCTGGGTGCTTTTAA-3’
ii. 3’ –TACCGACCCACGAAAATT-5’
i. 3'-UACCGACCCACGAAAATT-5' (mRNA)
ii. Met Ala Gly Cys Phe (protein)
i. The mRNA sequence synthesized from the bottom strand of the DNA template would be:
5’-AUGGCUGGGUGCUUUUAA-3’
The translated protein sequence using the Genetic Code would be:
Met-Ala-Gly-Cys-Phe-Stop
ii. In this case, the given sequence is already the template strand, so we can use it directly as the mRNA transcript without any modification.
5’-UACCGACCCACGAAAATT-3’
The translated protein sequence using the Genetic Code would be:
Tyr-Arg-Pro-Thr-Lys-Stop