A group of researchers isolate ‘Protein X’ from the wall of a human
stomach with the intent of learning how to synthesize stomach tissue
in the lab. Subsequently, they determine the exact sequence of amino
acids of the protein in its unfolded state, and create a functional
mRNA template to translate Protein X in vitro. They manage to
translate an exact copy of the polypeptide chain in the lab, but then
realize that it takes several days for the protein to fold into its final
tertiary structure. In vivo, they observe that several thousand copies
of Protein X are folded from polypeptide chains every minute. What
is NOT a plausible explanation for this difference in folding times?
a. The in vitro study lacks key enzymes
b. The temperature in vitro is too low
c. The in vitro study lacks key tRNA molecule
d. The in vitro PH is too hig