Write a summary on the aim of the journal article 'Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins' written by Ana Cristina Puhl, Cecilia Giacomini, and Gabriela Irazoqui.
The aim of the journal article 'Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins' is to develop a method for efficiently removing the histidine tag from recombinant proteins. The authors describe the covalent immobilization of tobacco-etch-virus NIa protease to a solid support, and demonstrate that this immobilized protease can effectively cleave histidine tags from recombinant proteins. They validate the effectiveness of this tool through a series of experiments, and suggest that it could be a useful tool for the production of purified recombinant proteins for downstream applications.
