A Sample had reagent solutions used: Iodine solution (no change), Biuret solution (pink), Benedict's solution (yellow) and Pure water (control, no change).
There were 2 colour changes.
The sample was found to contain trace amounts of glucose and amino acids. Based on the colour changes observed for this sample, provide a Biochemical explanation for these findings.
Demonstrate biochemistry-based principles behind reagent tests (that can result in more than one observable colour change depending on the amount of solute in the sample).
See your other post.
Can you help with this one? I do not know it.
There are two colour changes in this one:
Biuret solution (pink), Benedict's solution (yellow)
The sample was found to contain trace amounts of glucose and amino acids. Based on the colour changes observed for this sample, provide a Biochemical explanation for these findings.
Demonstrate biochemistry-based principles behind reagent tests (that can result in more than one observable colour change depending on the amount of solute in the sample).
A pink biuret test indicates shorter polypeptide chain.
A yellow Benedict's test means 0.5-1.0% sugar is present.
What biochemistry-based principles behind reagent tests results in more than one observable colour change?
(depending on the amount of solute in the sample)
I am not an organic chemist; I don't know that answer.
To understand the biochemical explanation for the color changes observed in this sample, we need to examine the reagent tests used and their specific reactions with glucose and amino acids.
1. Iodine solution: Iodine solution is commonly used to test for the presence of starch. It reacts with starch to form a dark blue-black complex. In this case, since there was no color change observed with iodine solution, it suggests that there was no significant amount of starch present in the sample.
2. Biuret solution: Biuret solution is a reagent commonly used to test for the presence of proteins. It reacts with peptide bonds in proteins to form a violet or pink color complex. The observed pink color change indicates the presence of proteins or, more specifically, amino acids that can react with the biuret reagent.
3. Benedict's solution: Benedict's solution is a reagent used to detect reducing sugars such as glucose. It contains copper ions, which are reduced by the aldehyde or ketone group present in the glucose molecule, forming a colored precipitate. The color changes range from green to yellow to brick red, depending on the concentration of the reducing sugar. In this case, the observed yellow color change indicates the presence of glucose.
4. Pure water (control): Pure water is used as a control to ensure that any observed changes in color are due to the presence of specific substances in the sample being tested.
Based on the color changes observed, the sample contains trace amounts of glucose (indicated by the yellow color change with Benedict's solution) and amino acids (indicated by the pink color change with Biuret solution). Glucose is a reducing sugar that reacts with Benedict's solution, while amino acids react with the biuret reagent and produce a pink color.
It's important to note that the intensity of the color change in both reagent tests can vary depending on the concentration of the solute (glucose or amino acids) present in the sample. Higher concentrations can result in more pronounced color changes. Therefore, the degree of color change can provide insight into the relative concentration of these solutes in the sample.