Friday
December 19, 2014

Homework Help: Biology

Posted by Jenna on Monday, August 4, 2014 at 3:43pm.

Consider the following 45 base-pair (bp) DNA sequence:

1 10 20 30 40
| . | . | . | . | .
5-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3
|||||||||||||||||||||||||||||||||||||||||||||
3-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5

Tip: If you want to see a review of PCR, we recommend this amazing animation and virtual lab made by the University of Utah.

You also have a collection of short DNA primers:

Primer 1: 5-CGTGGA-3
Primer 2: 5-TGTGTT-3
Primer 3: 5-ATCCAA-3
Primer 4: 5-CCTTGG-3
You put together several reaction mixtures, run them in the thermocycler under conditions that should produce a product if the reaction mixture is correct.

All mixtures contain:

5-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTG
GATCCAAGGC-3

The 45 bp DNA fragment
DNA polymerase that can function at high temperature
The necessary buffer conditions
For each mixture, indicate whether or not the PCR reaction would produce a product. If a product would be produced, give the length of that product in base-pairs (bp); if no product would be produced, give zero 0 for the length.



1
Added: dATP, dGTP, dCTP, Primer 2, Primer 4
- unanswered

2
Added: dATP, dGTP, dCTP, dTTP, Primer 2
- unanswered
3
Added: dATP, dGTP, dCTP, dTTP, Primer 2, Primer 4
- unanswered
4
Added: dATP, dGTP, dCTP, dTTP, Primer 1, Primer 3
- unanswered
5
Added: dATP, dGTP, dCTP, dTTP, Primer 2, Primer 3

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