Mutant C: Suppose an A/T base pair was inserted in between the two G/C base pairs at positions 18 and 19, marked by the letters above and below the strands. What would the amino acid sequence of the resulting protein be?
Be sure to enter the sequence N- to -C from left to right. Do not include the "N-" or "-C". Please use the 3-letter amino acid abbreviations (for example, "Ala-Gly-Ser"). We will ignore spaces and dashes when we grade your answer.
To determine the amino acid sequence of the resulting protein after the mutation, we need to translate the altered DNA sequence. Here is the step-by-step process:
1. Start by identifying the codon that contains the mutation. In this case, the mutation involves the insertion of an A/T base pair between the two G/C base pairs at positions 18 and 19.
2. Determine the codon affected by the mutation. The codon affected by the mutation is the one that contains the inserted base pair. In this case, it is the codon at positions 19 and 20.
3. Find the amino acid encoded by the original codon. To do this, refer to the genetic code chart. The codon that corresponds to positions 18 and 19 is "GCT" or glycine (Gly).
4. Determine the amino acid encoded by the inserted A/T base pair. In this case, the inserted A/T base pair does not form a complete codon. Therefore, it does not code for an amino acid.
5. Continue translating the DNA sequence after the insertion. After the inserted A/T base pair, we can resume translating the DNA sequence as usual.
6. Identify the codons after the mutation. Refer to the genetic code chart to determine the amino acids encoded by the codons after the mutation. Translate each codon by matching it with the appropriate amino acid.
Putting it all together, the resulting amino acid sequence after the mutation would begin with "Gly-" (glycine) followed by the amino acids encoded by the codons after the mutation.
Note: Without the original DNA sequence, we cannot provide the specific amino acid sequence after the mutation. This answer provides a general approach to determining the amino acid sequence after a mutation involving the insertion of an A/T base pair.
To determine the amino acid sequence of the resulting protein, we need to identify the codons affected by the mutation and translate them into their corresponding amino acids.
First, let's identify the affected codon. The mutation states that an A/T base pair was inserted between the two G/C base pairs at positions 18 and 19. Without the mutation, positions 18 and 19 would have had the codon "GGC" or "Gly" (glycine).
The insertion of the A/T base pair between them would result in the following sequence:
Original codon: GGC
Mutated codons: GAT and TGC
Now, let's determine the amino acid sequence for each codon:
GAT - Asp (aspartic acid)
TGC - Cys (cysteine)
Putting it all together, the resulting amino acid sequence would be "Asp-Cys" in 3-letter amino acid abbreviations.