1. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at
  2. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at
  3. Biochemistry

    An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at the
  4. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at
  5. chemistry lab

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at
  6. chemistry

    This is my third time to ask this and no one has answered. Please help. An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002
  7. Biochemistry

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for Tris
  8. chemistry Please Help Dr.BOB

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme (i.e., esterase)-catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for
  9. Biochemistry

    You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?
  10. chemistry

    what is the pH of the buffer when 4ml of 1M HCl is added to a 100ml buffer solution made using 5ml 1M Na2HPO4 and 5ml 1M NaH2PO4? The pKa for H+ + HPO4- ==> H2PO4 is 6.82. What would happen to the pH if the 100ml buffer had been made up at the starting
  11. Molecular Bio

    You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM MgCl2, and 0.025 % TritonX-100.
  12. Chemistry

    At body temperature (37C), K of an enzyme-catalyzed reaction is 2.3x10^14 times greater than k of the uncatalyzed reaction. Assuming that the frequency factor A is the same for both reaction, by how much does the enzyme the E^a?
  13. Chemistry

    In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, often abbreviated as Tris. At 25 C, Tris has a pKb of 5.91. The hydrochloride can be abbreviated as Tris HCl. What volume of 10.0 M NaOH is
  14. Biochemistry

    How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with 2.219 total
  15. chemistry

    Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 10-6 for the following aqueous reaction. (HOCH2)3CNH2 + H2O (HOCH2)3CNH3+ + OH -
  16. Biochem

    Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)
  17. biochemistry

    how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175
  18. Chemsitry

    Tris is an abbreviation for tris (hydroxymethyl) aminomethane, which is a weak base. Calculate how many grams of tris (mw=121.14g/mol) are needed to make 25mL of 0.10M tris solution. Calculate how many mL of 0.25M HCl are needed to convert half of the
  19. Chemistry

    One of the concerns about the use of Freons is that they will migrate to the upper atmosphere, where chlorine atoms can be generated by the following reaction: CCl2F2 --> CF2Cl + Cl Chlorine atoms can act as a catalyst for the destruction of ozone. The
  20. Biology

    Okay so if i have two enzyme catalyzed reactions: 1) delta g of the ES = -22kJ/mol 2) delta g od the ES = 42 kJ/mol Is this first reaction going to have a higher reaction rate because the of the negative delta g, because doesnt a negative delta g give you
  21. chemistry

    At body temperature (37°C), the rate constant of an enzyme-catalyzed decomposition is 3.0 × 108 times that of the uncatalyzed reaction. If the frequency factor, A, is the same for both processes, by how much does the enzyme lower the Ea?
  22. chemistry

    Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5. Useful information: pKa Tris = 8.1 Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1 Formula weight of Tris hydrochloride (acid form) = 158.0
  23. chemistry

    In protein precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the buffer solutions is best fitted for the said purpose?justify your answer. a. acetate buffer with pka=4.73? b. tris-aminomethane
  24. Biochemistry

    This is my last question and I just am not getting it for some reason. Prepare a 500 ml of 0.15 M tris buffer, pH 8.25 from tris mono hydrochloride, 0.400 M HCL, and 0.350 M NaOH. Determine amounts needed and them write the recipe. Pka for tris is 8.00
  25. biochemistry lab

    A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? B) If you add 2 mL of 1M NaOH to the solution in part A, what will be the pH? If it is needed, here is some addition information: TRIS pka= 8.3 mw of TRIS-HCL= 157.6
  26. Chemistry

    1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL
  27. Chemistry

    1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL
  28. Biochemistry

    Calculate the amount (in grams) of Tris Base needed to make 1L of 0.01M Tris buffer? Tris Base=121.4 g/mol
  29. Chemistry

    Starting with solid Tris base (FW 121.1) and 1 M HCl, describe how you would make 2 liters of 200 mM Tris-HCl buffer pH 7.5. pKa of Tris 8.14
  30. BioChem

    How much Tris-HCl would you need (in grams) to add to create 100 mL of a 0.223 M Tris buffer that is pH 9.64. The pKa of Tris-HCl is 8.30. I'm on my last try and thought I had it multiple times but keep getting it wrong.
  31. chemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated by ammonium sulfate
  32. Chemistry - acid base

    What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl? ( Tris. At 25 ∘C, Tris has a pKb of 5.91 ) a) The buffer from Part A is diluted to 1.00 L. To half of it (500. mL), you add 0.0250 mol of hydrogen ions
  33. Chemistry

    biochemists consider the citric acid cycle to be the central reaction sequence in metabolism. One of the key steps is an oxidation catalyzed by the enzyme isocitrate dehydrogenase and the oxidizing agent NAD+. Under certain conditions, the reaction in
  34. biology

    The conversion of molecule S to product P has a Keq = 2.0. Two different, but related enzymes, A and B, can catalyze the reaction. The product P serves as an allosteric inhibitor of enzyme B, but not of enzyme A. A. 10 mM S is placed into three separate
  35. Chem 111

    A solution is prepared by mixing 499 mL of 0.19 M Tris·Base and 592 mL of 0.37 M Tris·Acid. What is the pH of this solution? [Ka(Tris·Acid) = 8.5 × 10-9] Round your answer to two places past the decimal. I can do basic pH questions but I am at a loss
  36. Chemistry

    Calculate the pH of a solution prepared by dissolving 10.0 g of tris(hydroxymethyl)aminomethane ("tris base") plus 10.0 g of tris hydrochloride in 0.250 L of water. (a) What will be the pH if 10.5 mL of 0.500 M NaOH is added?
  37. biochemistry

    You have a 100 mMoles of A in the 10 ml reaction mixture which contains buffer and enzyme. The reaction is allowed to reach equilibrium at 25 o C and you measure the concentration of B as 10 mM. What is the delta Go' for the reaction in KJ.
  38. chemistry

    If you mixed equal volume of .1 M HCl and .20 M Tris (pka=8.3), IS THE RESULTING SOLUTION A BUFFER SOLUTION? Why? or Why not?
  39. This is grade 12 biology

    What is the effect of enzyme concentration on enzyme activity? Explain how much enzyme activity changes as enzyme concentration decreases, and discuss why this occurs (on a molecular level). Is this right (and can you add more): As enzyme concentration
  40. Chemistry 3A

    You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the
  41. chem biochem bio

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution???
  42. BCH

    How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is 121, mw of Hcl is 157.
  43. chemistry

    Prepare 150 ml of a reaction buffer containing 0.75% MgCl2, 35mM glucose, 750 nM ATP, and 20 mM Tris-Cl, pH 7.5. In addition, you have a 20% MgCl2 stock solution and the lab freezer has 10 mM ATP that you can dilute to the correct concentration.
  44. Biochemistry

    You wish to make 3 reactions (1 ml each) with the specified amounts of protein. The remainder of each reaction consists entirely of buffer. The source of your protein is a stock solution that has a concentration of 0.5 mg/ml. What is the volume of stock
  45. biochem

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution: 0.2M
  46. chemistry

    an ezyme -catalysed reaction was carried out in a solution buffered with 0.05 M phosphate ph 7.2 . As a result of the reaction ,0.06M of acid was formed. (phosphoric acid has three pka values. The one required for this calculation is pka2=7.2). 1) what was
  47. Chemistry

    One enzyme- catalyzed reaction in a biochemical cycle has an equilibrium constant (K1) that is 10 times the equilibrium constant (K2) of a second reaction. If the standard Gibbs energy of the former reaction is - 300 kJ mol-1, what is the standard reaction
  48. Chemistry

    A reaction proceeds with ∆ H = 40 kJ/mol. The energy of activation of the uncatalyzed reaction is 120 kJ/mol, whereas i t is 55 kJ/mol for the catalyzed reaction. How many times faster is the catalyzed reaction than the uncatalyzed reaction at 25°C?
  49. Chemistry

    Describe how a buffer behaves. Your description should include an explanation of why the addition of NaOH to the HAc solution formed a buffer. What happens to the pH when a small quantity of a strong acid or base is added to a buffer solution? What happens
  50. chemistry

    I am trying to calculate the how amount of Tris-bicarbonate should be added in the reaction. I would like to have final volume of bicarbonate 10mM in 2ml reaction. I made Tris-base (1M, 100ml) and instead of HCl, CO2 was bubbling to set pH7.5. 0.1L*0.1M=
  51. Bio

    How much solute is required to make 600mL of a 0.4 M solution of Tris buffer? FW of Tris Buffer= 121.1g Work: Molarity equation= moles of solute/ liters of solution Step 1: 121.1 g / 121.14 g/mol TB = 0.9996 mol TB Step 2: Molarity Eq'n= moles of solute/
  52. Chemistry

    A first order reaction has an activation enery of 50.0kJ/mol. When a catalyst is added the activation energy of the catalyzed reaction is Ea/2. Calculate the ration of k(uncatalyzed)/k(catalyzed) at 25 degree C.
  53. BIOCHEMISTRY

    Mark each statment that is true or false and give reasons. you will get credit only if your reasoning is correct a) at a steady state of an enzyme catalyzed reaction, there is not net change in [S] or [P] b) the linear anomer of beta-glucose is the more
  54. Biology

    Tube # 1: 1 ml of water, 3 ml of glucose-1-phosphate and 3 ml of Enzyme. Tube # 2: 1 ml of Ph 7 buffer, 3 ml of glucose-1-phosphate and 3 ml of Enzyme. Both of these reactions tested positive, and the same enzyme was used for both tubes. Why Did you get
  55. mym

    what are the factors that affect the rate of enzyme-catalyzed reactions in different liver parts ? For example, why does the reaction occur faster in lamb liver than chicken liver ?
  56. chemistry

    A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? (pka of TRIS: 8.3, molecular wt: 121.1 g) B) if you add 2mL of 1 M NaOH to the solution, what will be the pH? Thank you.
  57. chemistry please help

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the velocity of the reaction in terms of the
  58. biochemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the velocity of the reaction in terms of the
  59. CHEMISTRY

    1× SDS Running Buffer For this exercise, you will need to prepare 1 L of 1x SDS Running Buffer. The final composition of this buffer is: 25 mM Tris, 200 mM Glycine pH 8.3, 0.1% (w/v) SDS 1. To a 1 L beaker, add 500 ml of distilled water. Place a magnetic
  60. Chemistry

    A pH = 7.6 buffer is needed in the lab. This buffer is made by first dissolving 17.42 g K2HPO4 in 600 mL of water. What is the pH of this salt solution? This solution of course will be too basic becuase we only have the base of the buffer present. What
  61. chemistry

    The enzyme Y catalyzes the elementary reaction AB-->A + B An enzyme concentration of 1.0M Y can convert a maximum of 0.5M AB to the products A and B per second. What will happen when each of the following is added to a solution that contains 1.0M Y and
  62. chemistry lab

    Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka =
  63. chemistry

    Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka =
  64. Biology

    You have a stock solution with following components: glucose: 44 g/L NaCl: 191 g/L Tris Buffer: 275 g/L You mix 71 mL of the solution with water and bring the resulting solution to a final volume of 750 mL. What is the final concentration of glucose in
  65. Kim

    In an experiment to show the relationship between substrate concentration and activity of enzymes, why do we add a 0.5 of a 5 pH buffer solution to the enzyme test groups? the test tubes that we use to stop the reaction at specific times are basic
  66. science - chemistry pH problems

    Here is the hint I received on how to solve those tough pH problems I posted earlier: Both of these can be solved using the H-H and doing the algebra. The math itself is hard to type out, but in Q1 the values of [NaA] and [HA] are 0.04805 and 0.15195. In
  67. 9th Grade Biology

    You set up 5 samples that contained a range of sucrase concentrations but the same amount of substrate (sucrose). - The pH was kept constant in all tubes buy using the same amount of buffer. - The temperature was kept constant in all tubes (room temp) - A
  68. chemistry

    How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM? How much water?
  69. science

    Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases?
  70. chemistry

    From a 1M Tris solution, how is a 400ml of 0.2 M Tris prepared? I have no idea where to start
  71. Chemistry

    A typical buffer used by biochemists is called Tris, which can be depicted as R3N. A buffer that is prepared by mixing 500ml of .05M R3N with 500ml of .05MR3NHCl at 25 C has a pH of 8.40. If this same buffer is placed in a cold room at 4 C the pH increases
  72. Chemistry

    Assume you have prepared 100.0 mL of a buffer solution using 0.400 mol of acetic acid (pKa = 4.74) and 0.400 mol of sodium acetate. The pH of this buffer solution is initially 4.74. After preparing this buffer solution, you added 55.0 mL of a 1.10 M NaOH
  73. biology

    an experiment, a scientist notices that an enzyme’s catalytic rate decreases when the pH of the environment is changed. Which of the following best summarizes the effect of pH on the enzyme? A. pH changes the shape of the enzyme. B. pH takes energy away
  74. science

    1. In an experiment, a scientist notices that an enzyme’s catalytic rate decreases when the pH of the environment is changed. Which of the following best summarizes the effect of pH on the enzyme? A. pH changes the shape of the enzyme. B. pH takes energy
  75. chemistry

    A reaction produced 0.40 mmol of H in a buffered solution which contained 300ml of 0.06M succinate buffer, pH is 4. What was the pH of the medium after the reaction was completed? The pKa of succinate buffer is 4.21
  76. chemistry please help

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg
  77. Chemistry

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg
  78. Chemistry

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg
  79. physics/chem

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg
  80. biology

    another way to afect the enzyme is lowering the pH of the solution. However, lactase is supposed to be able to work in the stomack. Would lowering the pH of the enzyme solution affect the enzyme? Why or why not?
  81. Science

    You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the soln. after autoclaving
  82. science

    This is the hypothesis about the affect of inhibitors on enzyme: --If the competitive inhibitor presence, then it might cause the reaction to slow down due to some of the available enzyme sites are occupied by the inhibitor. --For noncompetitive inhibitor:
  83. Advance Biology

    Briefly summarize the differences between the words or phrases in each of the following. A reaction without an enzyme and a reaction with an enzyme.
  84. Need guidance on how to solve Michaelis Menten

    The question is below. If someone could at least give me an idea of where to start, or some steps of how I should go about solving this problem, I would be so extremely grateful. Thank you! You isolate an enzyme from teddy bear liver. The enzyme catalyzes
  85. chemistry

    200X buffer is given in class.How much of stock sol and how much distilled water is used to make? a)50 ml of a 1X buffer solution? b)100 ml of a 20X buffer solution? c)10 ml of a 400X solution
  86. chemistry

    When the reaction is run in a buffer solution, however, it is pseudo-first-order in CH3I. rate = k[CH3] What is the half-life of this reaction in a pH 10.00 buffer if the pseudo-first-order rate constant for this reaction is 7.3 x10-9 s-1?
  87. Chemistry

    You need to prepare 1.0 L of a buffer with a pH of 9.15. The concentration of the acid in the buffer needs to be 0.100 M. You have available to you a 1.00 M NH4Cl solution, a 6.00 M NaOH solution, and a 6.00 M HCl solution. Determine how to make this
  88. Biochem

    Desribe the preparation of 2.00 L of 0.100M pH 9.00 glycine starting with the sodium chloride salt of zwitterionic glycinhe and 1.00M NaOh. If a reaction that generated 2.00 mmoles of base were carried out in 100 mL of the above buffer, what would be the
  89. Chem

    An electrochemical cell based on the decomposition of H2O2 can be constructed based on the half-reactions in the table below. Half-Reaction | Standard Reduction Potential, E° H2O2+ 2e- => 2 OH- | 0.88 V O2+ 2 H2O + 2e- => H2O + 2 OH- | -0.15 V (d)
  90. Chemistry

    A 1X Phosphate Buffered Saline solution was prepared by adding 1.44g of Na2HPO4 and 1.44g of H2PO4- to 800mL of water. Additional components were dissolved in the solution: 8g of NaCl, 0.2g of KCl and 0.24g KH2PO4. The resulting buffer solution had a pH of
  91. chemistry

    Calculating final concentrations. Your instructor asks you to add the specified quantities of 2M Tris pH 8.0 buffer to 140 ml of distilled water. Calculate the final concentration and dilution factor of each solution. a)10ml b)20ml c)140ml
  92. biochemistry with more info that was needed

    Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer? Buffer pKa1 pKa2 pKa3
  93. biology

    1. Enzyme competition occurs when: A. one type of enzyme reacts with one type of substrate. B. an enzyme does not react with a substrate. C. three different types of enzymes react with one type of substrate. D. an enzyme stops a reaction. is it C 2. The
  94. chemistry

    If you add 5.0 mL of 0.50 M NaOH solution to 20.0 mL to Buffer C, what is the change in pH of the buffer? (where buffer C is 8.203 g sodium acetate with 100.0 mL of 1.0 M acetic acid) I have calculated the pH of buffer C to be 4.74. Now what? =\
  95. CHEMISTRY HELPPPP!!!!!!!!!

    Consider the decomposition of NH3 (g). 2 NH3 (g) → N2 (g) + 3 H2 (g) The reaction is catalyzed on a surface of either tungsten or osmium. The activation energies for the catalyzed and uncatalyzed reactions are given below. W Ea = 163 kJ/mol Os Ea =
  96. biochemistry

    The hydrolysis of one mole of sulphate ester produces one mole of protons.In a reaction tube containing 1.0ml of 0.02M TRIS buffer,pH8.08,sulphate ester was incubated with sulphatase for 10 min.At the end of reaction,the pH was reduced to 7.97.Calculated
  97. Chemistry

    How to make 100 mL of 100mM of Tris HCl and 100 mL of 100mM of Tris Base. Follow the pH of the resulting combined solution as it is heated from 4 to 50 degrees Celsius. I did not use the HH equation in this problem. I started with just converting to moles
  98. Chem--buffers

    Explain why a mixture formed by mixing 100 mL of 0.100M CH3COOH and 50 mL of 0.100M NaOH will act as a buffer? in adittion to this, how do you identify if a an aqueous solution is a buffer solution, such as a solution with an acid and a base, but no common
  99. math

    calculation of prepaired tris buffer 0.02mol/lit , ph 9.0
  100. chemistry

    who prepaire tris buffer 0.2 mmol/lit calculation