1. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at

    asked by alan on February 5, 2008
  2. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at the start

    asked by pankaj on July 9, 2015
  3. Biochemistry

    An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at the start of

    asked by Christine on June 26, 2016
  4. chemistry lab

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at

    asked by alan on February 3, 2008
  5. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at

    asked by alan on February 2, 2008
  6. chemistry

    This is my third time to ask this and no one has answered. Please help. An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002

    asked by alan on February 3, 2008
  7. Biochemistry

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for Tris

    asked by Melissa on February 7, 2011
  8. chemistry Please Help Dr.BOB

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme (i.e., esterase)-catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for

    asked by Kim on February 8, 2011
  9. Biochemistry

    You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?

    asked by Alberto on July 3, 2017
  10. Molecular Bio

    You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM MgCl2, and 0.025 % TritonX-100.

    asked by D. on February 13, 2014
  11. chemistry

    what is the pH of the buffer when 4ml of 1M HCl is added to a 100ml buffer solution made using 5ml 1M Na2HPO4 and 5ml 1M NaH2PO4? The pKa for H+ + HPO4- ==> H2PO4 is 6.82. What would happen to the pH if the 100ml buffer had been made up at the starting pH

    asked by matt on August 23, 2010
  12. Chemistry

    At body temperature (37C), K of an enzyme-catalyzed reaction is 2.3x10^14 times greater than k of the uncatalyzed reaction. Assuming that the frequency factor A is the same for both reaction, by how much does the enzyme the E^a?

    asked by Munavvar on February 28, 2015
  13. Chemistry

    In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, often abbreviated as Tris. At 25 C, Tris has a pKb of 5.91. The hydrochloride can be abbreviated as Tris HCl. What volume of 10.0 M NaOH is

    asked by Alexandra on November 14, 2010
  14. Biochemistry

    How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with 2.219 total

    asked by Rose on January 6, 2011
  15. chemistry

    Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 10-6 for the following aqueous reaction. (HOCH2)3CNH2 + H2O (HOCH2)3CNH3+ + OH -

    asked by John on March 11, 2009
  16. Biochem

    Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)

    asked by bugbabe623 on September 11, 2008
  17. biochemistry

    how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175

    asked by Anonymous on November 25, 2016
  18. Chemsitry

    Tris is an abbreviation for tris (hydroxymethyl) aminomethane, which is a weak base. Calculate how many grams of tris (mw=121.14g/mol) are needed to make 25mL of 0.10M tris solution. Calculate how many mL of 0.25M HCl are needed to convert half of the

    asked by Cassandra on April 3, 2012
  19. Chemistry

    One of the concerns about the use of Freons is that they will migrate to the upper atmosphere, where chlorine atoms can be generated by the following reaction: CCl2F2 --> CF2Cl + Cl Chlorine atoms can act as a catalyst for the destruction of ozone. The

    asked by Jim on October 27, 2010
  20. chemistry

    At body temperature (37°C), the rate constant of an enzyme-catalyzed decomposition is 3.0 × 108 times that of the uncatalyzed reaction. If the frequency factor, A, is the same for both processes, by how much does the enzyme lower the Ea?

    asked by sr734 on September 28, 2017
  21. Biology

    Okay so if i have two enzyme catalyzed reactions: 1) delta g of the ES = -22kJ/mol 2) delta g od the ES = 42 kJ/mol Is this first reaction going to have a higher reaction rate because the of the negative delta g, because doesnt a negative delta g give you

    asked by Cindy on January 27, 2013
  22. chemistry

    In protein precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the buffer solutions is best fitted for the said purpose?justify your answer. a. acetate buffer with pka=4.73? b. tris-aminomethane

    asked by jen on August 26, 2018
  23. chemistry

    Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5. Useful information: pKa Tris = 8.1 Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1 Formula weight of Tris hydrochloride (acid form) = 158.0

    asked by steven on September 26, 2012
  24. Biochemistry

    This is my last question and I just am not getting it for some reason. Prepare a 500 ml of 0.15 M tris buffer, pH 8.25 from tris mono hydrochloride, 0.400 M HCL, and 0.350 M NaOH. Determine amounts needed and them write the recipe. Pka for tris is 8.00

    asked by Kaylee on August 25, 2012
  25. biochemistry lab

    A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? B) If you add 2 mL of 1M NaOH to the solution in part A, what will be the pH? If it is needed, here is some addition information: TRIS pka= 8.3 mw of TRIS-HCL= 157.6

    asked by erin on February 2, 2008
  26. Chemistry

    1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL

    asked by Erin on April 8, 2013
  27. Chemistry

    1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL

    asked by Erin on April 8, 2013
  28. Chemistry

    Starting with solid Tris base (FW 121.1) and 1 M HCl, describe how you would make 2 liters of 200 mM Tris-HCl buffer pH 7.5. pKa of Tris 8.14

    asked by Brittnay on March 12, 2013
  29. BioChem

    How much Tris-HCl would you need (in grams) to add to create 100 mL of a 0.223 M Tris buffer that is pH 9.64. The pKa of Tris-HCl is 8.30. I'm on my last try and thought I had it multiple times but keep getting it wrong.

    asked by Kody on September 10, 2017
  30. Biochemistry

    Calculate the amount (in grams) of Tris Base needed to make 1L of 0.01M Tris buffer? Tris Base=121.4 g/mol

    asked by San on September 11, 2015
  31. chemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated by ammonium sulfate

    asked by stew on February 16, 2008
  32. Chemistry

    biochemists consider the citric acid cycle to be the central reaction sequence in metabolism. One of the key steps is an oxidation catalyzed by the enzyme isocitrate dehydrogenase and the oxidizing agent NAD+. Under certain conditions, the reaction in

    asked by Lety on June 20, 2014
  33. Chemistry - acid base

    What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl? ( Tris. At 25 ∘C, Tris has a pKb of 5.91 ) a) The buffer from Part A is diluted to 1.00 L. To half of it (500. mL), you add 0.0250 mol of hydrogen ions

    asked by K on March 9, 2018
  34. biology

    The conversion of molecule S to product P has a Keq = 2.0. Two different, but related enzymes, A and B, can catalyze the reaction. The product P serves as an allosteric inhibitor of enzyme B, but not of enzyme A. A. 10 mM S is placed into three separate

    asked by jay on October 15, 2007
  35. Chem 111

    A solution is prepared by mixing 499 mL of 0.19 M Tris·Base and 592 mL of 0.37 M Tris·Acid. What is the pH of this solution? [Ka(Tris·Acid) = 8.5 × 10-9] Round your answer to two places past the decimal. I can do basic pH questions but I am at a loss

    asked by Brittany on March 25, 2012
  36. Chemistry

    Calculate the pH of a solution prepared by dissolving 10.0 g of tris(hydroxymethyl)aminomethane ("tris base") plus 10.0 g of tris hydrochloride in 0.250 L of water. (a) What will be the pH if 10.5 mL of 0.500 M NaOH is added?

    asked by Sidney on May 28, 2016
  37. biochemistry

    You have a 100 mMoles of A in the 10 ml reaction mixture which contains buffer and enzyme. The reaction is allowed to reach equilibrium at 25 o C and you measure the concentration of B as 10 mM. What is the delta Go' for the reaction in KJ.

    asked by Sarah on July 25, 2012
  38. chemistry

    If you mixed equal volume of .1 M HCl and .20 M Tris (pka=8.3), IS THE RESULTING SOLUTION A BUFFER SOLUTION? Why? or Why not?

    asked by nicki on September 19, 2010
  39. chem biochem bio

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution???

    asked by mel on March 10, 2013
  40. This is grade 12 biology

    What is the effect of enzyme concentration on enzyme activity? Explain how much enzyme activity changes as enzyme concentration decreases, and discuss why this occurs (on a molecular level). Is this right (and can you add more): As enzyme concentration

    asked by Gilmore on February 28, 2008
  41. Chemistry 3A

    You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the

    asked by Katie on February 1, 2013
  42. chemistry

    Prepare 150 ml of a reaction buffer containing 0.75% MgCl2, 35mM glucose, 750 nM ATP, and 20 mM Tris-Cl, pH 7.5. In addition, you have a 20% MgCl2 stock solution and the lab freezer has 10 mM ATP that you can dilute to the correct concentration.

    asked by steven on September 25, 2012
  43. biochem

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution: 0.2M

    asked by Shea on March 10, 2013
  44. chemistry

    an ezyme -catalysed reaction was carried out in a solution buffered with 0.05 M phosphate ph 7.2 . As a result of the reaction ,0.06M of acid was formed. (phosphoric acid has three pka values. The one required for this calculation is pka2=7.2). 1) what was

    asked by josh on January 13, 2016
  45. BCH

    How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is 121, mw of Hcl is 157.

    asked by kishow on November 25, 2016
  46. Biochemistry

    You wish to make 3 reactions (1 ml each) with the specified amounts of protein. The remainder of each reaction consists entirely of buffer. The source of your protein is a stock solution that has a concentration of 0.5 mg/ml. What is the volume of stock

    asked by Alwxy on October 22, 2016
  47. Chemistry

    Describe how a buffer behaves. Your description should include an explanation of why the addition of NaOH to the HAc solution formed a buffer. What happens to the pH when a small quantity of a strong acid or base is added to a buffer solution? What happens

    asked by Terrell on March 14, 2013
  48. chemistry

    I am trying to calculate the how amount of Tris-bicarbonate should be added in the reaction. I would like to have final volume of bicarbonate 10mM in 2ml reaction. I made Tris-base (1M, 100ml) and instead of HCl, CO2 was bubbling to set pH7.5. 0.1L*0.1M=

    asked by chocho on June 27, 2016
  49. Chemistry

    One enzyme- catalyzed reaction in a biochemical cycle has an equilibrium constant (K1) that is 10 times the equilibrium constant (K2) of a second reaction. If the standard Gibbs energy of the former reaction is - 300 kJ mol-1, what is the standard reaction

    asked by harry on April 10, 2012
  50. Chemistry

    A first order reaction has an activation enery of 50.0kJ/mol. When a catalyst is added the activation energy of the catalyzed reaction is Ea/2. Calculate the ration of k(uncatalyzed)/k(catalyzed) at 25 degree C.

    asked by Darcy on April 8, 2010
  51. Bio

    How much solute is required to make 600mL of a 0.4 M solution of Tris buffer? FW of Tris Buffer= 121.1g Work: Molarity equation= moles of solute/ liters of solution Step 1: 121.1 g / 121.14 g/mol TB = 0.9996 mol TB Step 2: Molarity Eq'n= moles of solute/

    asked by Cindy on January 30, 2018
  52. BIOCHEMISTRY

    Mark each statment that is true or false and give reasons. you will get credit only if your reasoning is correct a) at a steady state of an enzyme catalyzed reaction, there is not net change in [S] or [P] b) the linear anomer of beta-glucose is the more

    asked by UCI KID on January 26, 2013
  53. mym

    what are the factors that affect the rate of enzyme-catalyzed reactions in different liver parts ? For example, why does the reaction occur faster in lamb liver than chicken liver ?

    asked by Am,y on January 11, 2010
  54. Chemistry

    A reaction proceeds with ∆ H = 40 kJ/mol. The energy of activation of the uncatalyzed reaction is 120 kJ/mol, whereas i t is 55 kJ/mol for the catalyzed reaction. How many times faster is the catalyzed reaction than the uncatalyzed reaction at 25°C?

    asked by Jessica on February 28, 2015
  55. chemistry

    A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? (pka of TRIS: 8.3, molecular wt: 121.1 g) B) if you add 2mL of 1 M NaOH to the solution, what will be the pH? Thank you.

    asked by matt on February 2, 2008
  56. Biology

    Tube # 1: 1 ml of water, 3 ml of glucose-1-phosphate and 3 ml of Enzyme. Tube # 2: 1 ml of Ph 7 buffer, 3 ml of glucose-1-phosphate and 3 ml of Enzyme. Both of these reactions tested positive, and the same enzyme was used for both tubes. Why Did you get

    asked by Brook on February 4, 2012
  57. chemistry

    The enzyme Y catalyzes the elementary reaction AB-->A + B An enzyme concentration of 1.0M Y can convert a maximum of 0.5M AB to the products A and B per second. What will happen when each of the following is added to a solution that contains 1.0M Y and

    asked by tomi on March 23, 2009
  58. chemistry please help

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the velocity of the reaction in terms of the

    asked by stew on February 16, 2008
  59. biochemistry

    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Calculate the velocity of the reaction in terms of the

    asked by stew on February 17, 2008
  60. CHEMISTRY

    1× SDS Running Buffer For this exercise, you will need to prepare 1 L of 1x SDS Running Buffer. The final composition of this buffer is: 25 mM Tris, 200 mM Glycine pH 8.3, 0.1% (w/v) SDS 1. To a 1 L beaker, add 500 ml of distilled water. Place a magnetic

    asked by Samari on March 20, 2018
  61. Chemistry

    A pH = 7.6 buffer is needed in the lab. This buffer is made by first dissolving 17.42 g K2HPO4 in 600 mL of water. What is the pH of this salt solution? This solution of course will be too basic becuase we only have the base of the buffer present. What

    asked by Carl on March 12, 2015
  62. science - chemistry pH problems

    Here is the hint I received on how to solve those tough pH problems I posted earlier: Both of these can be solved using the H-H and doing the algebra. The math itself is hard to type out, but in Q1 the values of [NaA] and [HA] are 0.04805 and 0.15195. In

    asked by ajones51 on October 11, 2006
  63. 9th Grade Biology

    You set up 5 samples that contained a range of sucrase concentrations but the same amount of substrate (sucrose). - The pH was kept constant in all tubes buy using the same amount of buffer. - The temperature was kept constant in all tubes (room temp) - A

    asked by Mellisa B on September 6, 2016
  64. Kim

    In an experiment to show the relationship between substrate concentration and activity of enzymes, why do we add a 0.5 of a 5 pH buffer solution to the enzyme test groups? the test tubes that we use to stop the reaction at specific times are basic

    asked by Bio on September 20, 2014
  65. chemistry

    Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka =

    asked by alan on February 2, 2008
  66. chemistry lab

    Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka =

    asked by ryan on February 3, 2008
  67. Biology

    You have a stock solution with following components: glucose: 44 g/L NaCl: 191 g/L Tris Buffer: 275 g/L You mix 71 mL of the solution with water and bring the resulting solution to a final volume of 750 mL. What is the final concentration of glucose in

    asked by Dee on February 11, 2015
  68. chemistry

    How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM? How much water?

    asked by Abass on July 8, 2013
  69. Chemistry

    A typical buffer used by biochemists is called Tris, which can be depicted as R3N. A buffer that is prepared by mixing 500ml of .05M R3N with 500ml of .05MR3NHCl at 25 C has a pH of 8.40. If this same buffer is placed in a cold room at 4 C the pH increases

    asked by Josh on November 17, 2011
  70. chemistry

    From a 1M Tris solution, how is a 400ml of 0.2 M Tris prepared? I have no idea where to start

    asked by Dave on September 18, 2011
  71. science

    Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases?

    asked by Ron on August 2, 2011
  72. Chemistry

    Assume you have prepared 100.0 mL of a buffer solution using 0.400 mol of acetic acid (pKa = 4.74) and 0.400 mol of sodium acetate. The pH of this buffer solution is initially 4.74. After preparing this buffer solution, you added 55.0 mL of a 1.10 M NaOH

    asked by J on November 26, 2015
  73. biology

    an experiment, a scientist notices that an enzyme’s catalytic rate decreases when the pH of the environment is changed. Which of the following best summarizes the effect of pH on the enzyme? A. pH changes the shape of the enzyme. B. pH takes energy away

    asked by hannah on November 7, 2013
  74. science

    1. In an experiment, a scientist notices that an enzyme’s catalytic rate decreases when the pH of the environment is changed. Which of the following best summarizes the effect of pH on the enzyme? A. pH changes the shape of the enzyme. B. pH takes energy

    asked by hannah on November 6, 2013
  75. Chemistry

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg

    asked by Amelia on November 11, 2009
  76. chemistry please help

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg

    asked by Amelia on November 10, 2009
  77. physics/chem

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg

    asked by Amelia on November 9, 2009
  78. Chemistry

    ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this i have 10 micro litres of a 5mg

    asked by Anonymous on November 11, 2009
  79. chemistry

    A reaction produced 0.40 mmol of H in a buffered solution which contained 300ml of 0.06M succinate buffer, pH is 4. What was the pH of the medium after the reaction was completed? The pKa of succinate buffer is 4.21

    asked by veronica on April 19, 2013
  80. Need guidance on how to solve Michaelis Menten

    The question is below. If someone could at least give me an idea of where to start, or some steps of how I should go about solving this problem, I would be so extremely grateful. Thank you! You isolate an enzyme from teddy bear liver. The enzyme catalyzes

    asked by Student on December 15, 2016
  81. Science

    You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the soln. after autoclaving

    asked by Sue on September 12, 2011
  82. science

    This is the hypothesis about the affect of inhibitors on enzyme: --If the competitive inhibitor presence, then it might cause the reaction to slow down due to some of the available enzyme sites are occupied by the inhibitor. --For noncompetitive inhibitor:

    asked by alma on September 16, 2008
  83. biology

    another way to afect the enzyme is lowering the pH of the solution. However, lactase is supposed to be able to work in the stomack. Would lowering the pH of the enzyme solution affect the enzyme? Why or why not?

    asked by sara on November 11, 2011
  84. chemistry

    200X buffer is given in class.How much of stock sol and how much distilled water is used to make? a)50 ml of a 1X buffer solution? b)100 ml of a 20X buffer solution? c)10 ml of a 400X solution

    asked by anonymous on August 28, 2012
  85. Chemistry

    You need to prepare 1.0 L of a buffer with a pH of 9.15. The concentration of the acid in the buffer needs to be 0.100 M. You have available to you a 1.00 M NH4Cl solution, a 6.00 M NaOH solution, and a 6.00 M HCl solution. Determine how to make this

    asked by Lily on February 25, 2018
  86. Biochem

    Desribe the preparation of 2.00 L of 0.100M pH 9.00 glycine starting with the sodium chloride salt of zwitterionic glycinhe and 1.00M NaOh. If a reaction that generated 2.00 mmoles of base were carried out in 100 mL of the above buffer, what would be the

    asked by Sandra on September 13, 2011
  87. Advance Biology

    Briefly summarize the differences between the words or phrases in each of the following. A reaction without an enzyme and a reaction with an enzyme.

    asked by Anonymous on October 3, 2009
  88. chemistry

    When the reaction is run in a buffer solution, however, it is pseudo-first-order in CH3I. rate = k[CH3] What is the half-life of this reaction in a pH 10.00 buffer if the pseudo-first-order rate constant for this reaction is 7.3 x10-9 s-1?

    asked by anglo on June 5, 2009
  89. biochemistry with more info that was needed

    Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer? Buffer pKa1 pKa2 pKa3

    asked by stew on January 27, 2008
  90. Chem

    An electrochemical cell based on the decomposition of H2O2 can be constructed based on the half-reactions in the table below. Half-Reaction | Standard Reduction Potential, E° H2O2+ 2e- => 2 OH- | 0.88 V O2+ 2 H2O + 2e- => H2O + 2 OH- | -0.15 V (d)

    asked by Jess on April 23, 2013
  91. chemistry

    Calculating final concentrations. Your instructor asks you to add the specified quantities of 2M Tris pH 8.0 buffer to 140 ml of distilled water. Calculate the final concentration and dilution factor of each solution. a)10ml b)20ml c)140ml

    asked by anonymous on August 28, 2012
  92. Chemistry

    A 1X Phosphate Buffered Saline solution was prepared by adding 1.44g of Na2HPO4 and 1.44g of H2PO4- to 800mL of water. Additional components were dissolved in the solution: 8g of NaCl, 0.2g of KCl and 0.24g KH2PO4. The resulting buffer solution had a pH of

    asked by Nicole on April 28, 2015
  93. biology

    1. Enzyme competition occurs when: A. one type of enzyme reacts with one type of substrate. B. an enzyme does not react with a substrate. C. three different types of enzymes react with one type of substrate. D. an enzyme stops a reaction. is it C 2. The

    asked by Amy on July 13, 2013
  94. CHEMISTRY HELPPPP!!!!!!!!!

    Consider the decomposition of NH3 (g). 2 NH3 (g) → N2 (g) + 3 H2 (g) The reaction is catalyzed on a surface of either tungsten or osmium. The activation energies for the catalyzed and uncatalyzed reactions are given below. W Ea = 163 kJ/mol Os Ea = 197

    asked by anon on March 12, 2015
  95. chemistry

    who prepaire tris buffer 0.2 mmol/lit calculation

    asked by rakesh on April 21, 2016
  96. math

    calculation of prepaired tris buffer 0.02mol/lit , ph 9.0

    asked by rakesh on April 21, 2016
  97. chemistry

    If you add 5.0 mL of 0.50 M NaOH solution to 20.0 mL to Buffer C, what is the change in pH of the buffer? (where buffer C is 8.203 g sodium acetate with 100.0 mL of 1.0 M acetic acid) I have calculated the pH of buffer C to be 4.74. Now what? =\

    asked by Chelsea on November 7, 2010
  98. biochemistry

    The hydrolysis of one mole of sulphate ester produces one mole of protons.In a reaction tube containing 1.0ml of 0.02M TRIS buffer,pH8.08,sulphate ester was incubated with sulphatase for 10 min.At the end of reaction,the pH was reduced to 7.97.Calculated

    asked by ameera on January 8, 2011
  99. Biology Chemistry Part

    I'm trying to calculated the rate of reaction In this lab I took a 50 mL beaker and poured in 10 ml of cold distilled water into it, I then poured in 30 ml of original enzyme solution (I believed we used cold potato juice or something), I then took a piece

    asked by Kate on September 26, 2010
  100. Chem--buffers

    Explain why a mixture formed by mixing 100 mL of 0.100M CH3COOH and 50 mL of 0.100M NaOH will act as a buffer? in adittion to this, how do you identify if a an aqueous solution is a buffer solution, such as a solution with an acid and a base, but no common

    asked by Rav on March 1, 2010