Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5. Useful information: pKa Tris = 8.1 Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1 Formula weight of Tris hydrochloride (acid form) = 158.0

47,952 results

chemistry

Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5. Useful information: pKa Tris = 8.1 Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1 Formula weight of Tris hydrochloride (acid form) = 158.0

Biochemistry

How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with 2.219 total [Tris}=(0.219/1.219)*0.1 M= 0.018 M...

Chemsitry

Tris is an abbreviation for tris (hydroxymethyl) aminomethane, which is a weak base. Calculate how many grams of tris (mw=121.14g/mol) are needed to make 25mL of 0.10M tris solution. Calculate how many mL of 0.25M HCl are needed to convert half of the moles of tris present in ...

Biochem

Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)

Chemistry

Calculate the pH of a solution prepared by dissolving 10.0 g of tris(hydroxymethyl)aminomethane ("tris base") plus 10.0 g of tris hydrochloride in 0.250 L of water. (a) What will be the pH if 10.5 mL of 0.500 M NaOH is added?

chemistry

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the ...

Biochemistry

Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for Tris (hydroxymethyl) ...

biochemistry

how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175

chemistry Please Help Dr.BOB

Complete hydrolysis of 25 mM ethyl acetate in an enzyme (i.e., esterase)-catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the abbreviation for Tris (hydroxymethyl) ...

chemistry

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the ...

chemistry lab

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the ...

Chemistry

Starting with solid Tris base (FW 121.1) and 1 M HCl, describe how you would make 2 liters of 200 mM Tris-HCl buffer pH 7.5. pKa of Tris 8.14

biochemistry lab

A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? B) If you add 2 mL of 1M NaOH to the solution in part A, what will be the pH? If it is needed, here is some addition information: TRIS pka= 8.3 mw of TRIS-HCL= 157.6 g/mol; mw TRIS (...

chemistry

This is my third time to ask this and no one has answered. Please help. An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced...

Biochemistry

This is my last question and I just am not getting it for some reason. Prepare a 500 ml of 0.15 M tris buffer, pH 8.25 from tris mono hydrochloride, 0.400 M HCL, and 0.350 M NaOH. Determine amounts needed and them write the recipe. Pka for tris is 8.00

BCH

How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is 121, mw of Hcl is 157.

Biochemistry

Calculate the amount (in grams) of Tris Base needed to make 1L of 0.01M Tris buffer? Tris Base=121.4 g/mol

Biochemistry

You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?

Chemistry

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, often abbreviated as Tris. At 25 C, Tris has a pKb of 5.91. The hydrochloride can be abbreviated as Tris HCl. What volume of 10.0 M NaOH is needed to prepare a ...

chemistry

Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 10-6 for the following aqueous reaction. (HOCH2)3CNH2 + H2O (HOCH2)3CNH3+ + OH - (HOCH2)3CNH2=TRIS (HOCH2)3CNH3+=...

Biochemistry

An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at the start of the ...

chemistry

. An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso (Conjugate base) at the start of the ...

BioChem

How much Tris-HCl would you need (in grams) to add to create 100 mL of a 0.223 M Tris buffer that is pH 9.64. The pKa of Tris-HCl is 8.30. I'm on my last try and thought I had it multiple times but keep getting it wrong.

chemistry

what is the pH of the buffer when 4ml of 1M HCl is added to a 100ml buffer solution made using 5ml 1M Na2HPO4 and 5ml 1M NaH2PO4? The pKa for H+ + HPO4- ==> H2PO4 is 6.82. What would happen to the pH if the 100ml buffer had been made up at the starting pH (6.82) using Tris...

Chem 111

A solution is prepared by mixing 499 mL of 0.19 M Tris·Base and 592 mL of 0.37 M Tris·Acid. What is the pH of this solution? [Ka(Tris·Acid) = 8.5 × 10-9] Round your answer to two places past the decimal. I can do basic pH questions but I am at a loss on this one. Please help.

chemistry

A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH? (pka of TRIS: 8.3, molecular wt: 121.1 g) B) if you add 2mL of 1 M NaOH to the solution, what will be the pH? Thank you.

chemistry

Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka = 8.3)? My answer: I used...

chemistry lab

Here are 2 questions I worked out, but need them checked. Also, can't get answer to another question correct unless question 1 is correct. Would you check the first 2 and help me with the third? 1) What is the pH of a 0.05 M solution of TRIS acid (pka = 8.3)? My answer: I used...

chemistry

if you mix 50ml of 0.1 tris acid with 60ml if 0.2m tris base, what will be the resulting pH?

chemistry

I am trying to calculate the how amount of Tris-bicarbonate should be added in the reaction. I would like to have final volume of bicarbonate 10mM in 2ml reaction. I made Tris-base (1M, 100ml) and instead of HCl, CO2 was bubbling to set pH7.5. 0.1L*0.1M= 0.01 moles of Tris...

Bio

How much solute is required to make 600mL of a 0.4 M solution of Tris buffer? FW of Tris Buffer= 121.1g Work: Molarity equation= moles of solute/ liters of solution Step 1: 121.1 g / 121.14 g/mol TB = 0.9996 mol TB Step 2: Molarity Eq'n= moles of solute/ liters of sol'n ... so...

chemistry

From a 1M Tris solution, how is a 400ml of 0.2 M Tris prepared? I have no idea where to start

Chemistry

How to make 100 mL of 100mM of Tris HCl and 100 mL of 100mM of Tris Base. Follow the pH of the resulting combined solution as it is heated from 4 to 50 degrees Celsius. I did not use the HH equation in this problem. I started with just converting to moles needed and then to ...

Chemistry 3A

You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer. I know this...

Chemistry

How to make 100 mL of 100mM of Tris HCl and 100 mL of 100mM of Tris Base. Follow the pH of the resulting combined solution as it is heated from 0 to 50 degrees Celsius.

chem biochem bio

You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution??? 0.2M NaCl 0.05M Tris, pH...

AP Chemistry

Write the formula for each of the following compounds, being sure to use brackets to indicate the coordination sphere. (b) bis(bipyridyl)cadmium(II) chloride (c) potassium tetrabromo(ortho-phenanthroline)- cobaltate(III) (d) cesium diamminetetracyanochromate(III) (e) tris(...

Chemistry

1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL of 0.18 M Tris·Base ...

Chemistry

1. Calculate the pH of a buffer solution that contains 0.32 M benzoic acid (C6H5CO2H) and 0.17 M sodium benzoate (C6H5COONa). [Ka = 6.5 × 10-5 for benzoic acid] Round your answer to two places past the decimal. 2. A solution is prepared by mixing 470 mL of 0.18 M Tris·Base ...

Science

You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the soln. after autoclaving is done. Assume that ...

biochemistry with more info that was needed

Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer? Buffer pKa1 pKa2 pKa3 formula wt.(g/mol) ...

Biochemistry

What is the pH of a 0.1M solution of TRIS in the acid from with pKa =8.3

biochem

You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the following solution: 0.2M NaCl 0.05M Tris, pH 8...

biochem

If I am given a solid chemical form of both the acid and the base, how do I determine how many grams of each I need to make 1L of a 25mM Tris-HCl buffer with acid concentration of 18.94mM, and base concentration of 6.06mM?

BIO

Can someone help with this question? Here are the formula weights for three compounds often used in molecular biology: NaCl, 58.44; Tris base, 121.1; EDTA, 372.2 a) For each of these three compounds, how much solute is required to make 1L of a 1M solution?

chemistry

If you mixed equal volume of .1 M HCl and .20 M Tris (pka=8.3), IS THE RESULTING SOLUTION A BUFFER SOLUTION? Why? or Why not?

Molecular Bio

You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM MgCl2, and 0.025 % TritonX-100. You search around ...

chemistry

what are the chemical formula for sodium tetrachloroplatinate(II) and tris(ethylenediamine)cobalt(III) bromide

math

calculation of prepaired tris buffer 0.02mol/lit , ph 9.0

chemistry

who prepaire tris buffer 0.2 mmol/lit calculation

Biology

The following chemicals have the indicated formula weight Surcose = 342.3g; Potassium dihydrogen=136.09; and Tris=121.14 Also the following solution already made: 20%SDS, 5M NaCl, 500mM EDTA. You have a 40% glycerol solution, and 10X TBE. List the step to make the following ...

Chemistry

How do you make Tris into the acid form?

Chemistry

How much Tris do you need to weigh out to make 250 ml of the 50 mM buffer? A. 0.95g B. 1.51g C. 2.42 D. 1.75g

chemistry

Prepare 150 ml of a reaction buffer containing 0.75% MgCl2, 35mM glucose, 750 nM ATP, and 20 mM Tris-Cl, pH 7.5. In addition, you have a 20% MgCl2 stock solution and the lab freezer has 10 mM ATP that you can dilute to the correct concentration.

Biochemisty

If I take 5mL of a TRIS/HCL buffer with a pH of 7.4 and add 1mL of 1M HCL, how do I determine the new pH?

chemistry

How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM? How much water?

Chemistry

A typical buffer used by biochemists is called Tris, which can be depicted as R3N. A buffer that is prepared by mixing 500ml of .05M R3N with 500ml of .05MR3NHCl at 25 C has a pH of 8.40. If this same buffer is placed in a cold room at 4 C the pH increases to 9.2. From this ...

Chemistry

Why is the equivalence point pH of the HCl-TRIS titration = 4.6?

Chem

What is the name of Co2(C2O4)3 is it cobalt (2) tris ethanedioate

chemistry

I asked this before, but got no response. A) If you make up a solution of 250 mL of 0.1 M TRIS in the acid form, what will be the pH? B) If you add 2 mL of 1 M NaOH to the solution in part A, what will be the pH? Thanks.

Biochem

Describe the steps (and the glassware used) to making 100 mL of a 1 M Tris pH 7.8.

Biochem

Describe the steps (and the glassware used) to making 100 mL of a 1 M Tris pH 7.8.

chemistry

Calculating final concentrations. Your instructor asks you to add the specified quantities of 2M Tris pH 8.0 buffer to 140 ml of distilled water. Calculate the final concentration and dilution factor of each solution. a)10ml b)20ml c)140ml

Chemistry

Robin the Research scientist prepares 100 mLs of a solution of 100 mM of Tris HCl. What is the pH? I am confused since we do not know the conjugate base concentration so how to solve for the pH

Molecular cell Biology

The following chemicals have the indicated formula weights:Sucrose-342.3,Potassium dihydrogen phosphate(KH2PO40-136.09,Tris -121.14.You also have the following solutions made:20%SDS,5M NaCl,500mM EDTA.You have a 40%glycerol solution and 10X TBE.List the steps to make the ...

chemistry

what are the precautions taken while preparing tris(acetylacetonato)-manganese 3 from potassium permanganate and acetylacetone.

Biology

You have a stock solution with following components: glucose: 44 g/L NaCl: 191 g/L Tris Buffer: 275 g/L You mix 71 mL of the solution with water and bring the resulting solution to a final volume of 750 mL. What is the final concentration of glucose in g/L?

Chemistry

The aquation of tris(1, 10-phenanthroline)iron(II) in acid solution takes place according to the equation: Fe(phen)3^2+ + 3H3O + 3H2O==> (Fe(H2O)6^2+ + 3 phenH^+ If the activation energy is 126 kJ/mol and frequency factor is 8.62 x 1017 s-1, at what temperature is the rate ...

Chemistry

Robin the Research scientist prepares 100 mLs of a solution of 100 mM of Tris HCl. What is the pH? and part two for this question Calculate the pH when the scientist adds 100 ul of 50% NaOH I am confused since we do not know the conjugate base concentration so how to solve for...

General chemistry

What is the name of the complex [Ni(en)3]3[Cr(CN)6]2? tris(ethylenediamine)nickel(II) hexacyanochromate(III) is this correct? im not sure if it should be II and III or the other way around

Chemistry

The aquation of tris(1,10-phenanthroline)iron(II) in acid solution takes place according to the equation: Fe(phen)32++ 3 H3O++ 3 H2O →Fe(H2O)62++ 3 phenH+. If the activation energy, Ea, is 126 kJ/mol and the rate constant at 30°C is 9.8 × 10-3 min-1, what is the rate ...

Chemistry

1. Write the formula for these compounds: a) sodium tris(oxalato)rhodium(III) b) sodium tetrachloroplatinate(II) c) triamminetrichloronickel(IV) sulfate For a) I put: Na3Rh(C2O4)3, however got an error message of this: "Your answer appears to be missing required square ...

chemistry

Robin the Research scientist prepares 100 mLs of a solution of 100 mM of Tris HCl. What is the pH? and part two for this question Calculate the pH when the scientist adds 100 ul of 50% NaOH

CHEMISTRY

how to calculate the weight of solute req'd to prepare 1 liter of 1 normal of: a.) LiOH b.) phosphoric acid my work: formula= gew/L or w/ewxL i get their mw then divide it to their H/OH so i can get EW then multiply it to 1. a.) 24 g/mol/1 b.) 98g/mol/3 i don't know what's ...

organic chem

. Use the Henderson-Hasselbach equation: pH = pKa + log [A-]/[AH], where A- is the conjugate base and AH is the weak acid. Calculate the pH of an acetate buffer in which the acetate concentration [CH3COO-] is 3.5 times greater than acetic acid [CH3COOH]. pKa = 4.74. 9. How ...

Chemistry

Dissolving one mole of salt in water yields two moles of ions. A:NH4[Pt(NH3)Cl3] B: tris(ethylenediamine)cobalt(III) sulfate C: tetraaquadichloronickel(IV) chloride D: [Co(NH3)2(en)Cl2]Cl E: [Co(en)2Cl2]Br F: sodium hexacyanoferrate(II) None of these compounds

Chemistry

The pH of a buffer can be predicted using the Hendersen-Hasselbach equation: pH=pKa+ log([conjugate base][conjugate acid]) The choice of the conjugate acid-base pair (as you did in the previous questions) determines the pKa value to be used in the H-H equation. By varying the ...

CHEMISTRY HELP

Calculate the amount (in mL) of 1.520M NaOH that is required to add the following acetic acid solution to prepare a buffer with the corresponding pH: 30.00mL of a 5.00% (w/v%) acetic acid; the resulting acetate buffer has a pH of 5.75. pKa of acetic acid = 4.74 So I got this: ...

biochemistry

The hydrolysis of one mole of sulphate ester produces one mole of protons.In a reaction tube containing 1.0ml of 0.02M TRIS buffer,pH8.08,sulphate ester was incubated with sulphatase for 10 min.At the end of reaction,the pH was reduced to 7.97.Calculated the amount of sulphate...

Chem

The coordination compound has five stereoisomers (geometric plus optical isomers). Select all that apply... A:NH4[Pt(NH3)Cl3] B: tris(ethylenediamine)cobalt(III) sulfate C: tetraaquadichloronickel(IV) chloride D: [Co(NH3)2(en)Cl2]Cl E: [Co(en)2Cl2]Br F: sodium hexacyanoferrate...

org. chem

How to start these problems out: Please help thank you Use the Henderson-Hasselbach equation: pH = pKa + log [A-]/[AH], where A- is the conjugate base and AH is the weak acid. Calculate the pH of an acetate buffer in which the acetate concentration [CH3COO-] is 3.5 times ...

Chemistry

I have the answer to this question but the book does not explain WHY this is a good buffer solution? It uses pH=pKa - log(base/acid) and shows 7.2=7.2 - log(1). I don't understand why they can only use pKa7.2 and ignore the pKa 12.7. You are instructed to prepare 100ml of a 0....

bio chem

Explain how to prepare 1L of a buffer at pH 7.4 using 1M hypochlorous acid (Ka = 3.5 x 10-8) and a 1 M NaOH solution. Indicate the volume of hypochlorous acid and sodium hydroxide to use. pH = pKa + log ([A-]/[HA]) Ka= [H+][A-]/[HA] Molarity = moles/L moles = g/molecular weight

Chemistry

If you need to prepare 250.0 mL of a pH 5.00 buffer that has a total buffer concentration of acetic acid + sodium acetate of 0.050M, how many moles of each will you need to prepare the solution? Given solutions of acetic acid and sodium acetate with concentrations of 0.10M and...

Biochemistry

Susie is instructed to prepare 400mL of a 0.3M acetate buffer of pH=4.44, and she is given sodium acetate trihydrate and glacial acetate acid (99% by weight, density=1.05 g/mL0. Susie can't figure out what to do! How should she prepare this buffer? Acetic acid, Ka=1.8x10^-5

chemistry

What is the pH of a solution prepared by dissolving 8.40 g of aniline hydrochloride C6H5NH3Cl in 750 mL of 0.210 M aniline,C6H5NH2? i kno to use the ph = pka + log (base/acid). getting pka isnt the issue, but what do i put into the log (base/acid)? (0.210/????) thanks

chemistry

What is the pH of a solution prepared by dissolving 8.40 g of aniline hydrochloride C6H5NH3Cl in 750 mL of 0.210 M aniline,C6H5NH2? i kno to use the ph = pka + log (base/acid). getting pka isnt the issue, but what do i put into the log (base/acid)? (0.210/????)

Chemistry

In a experiment to determine the molecular weight and the Ka for ascorbic acid (vit. c.) a student dissolved 1.3713g of the monoprotic acid in water to make 50 mL of solution. The pH was monitored throughout the titration. The equivalence point was reached when 35.23 mL of the...

Biochemistry

Calculate how you would make 0.5L of a 0.5M (500mM) acetate buffer at a pH of 4.7. Available is sodium acetate and glacial acetic acid. pH=4.7 pKa of acetic acid=4.76 Not sure if I did this next part right: 4.7=4.76 + log [base]/[acid] -0.06= log [base]/[acid] 10^-0.06= [base...

Analytical Chemistry

Suggest a range of sample masses for the indicated primary standard if it is desired to use between 35 and 45mL of titrant: (a) 0.175 M HClO4 titrated against Na2CO3(CO2 product) (b) 0.085 M HCl titrated against Na2C2O4 Na2C2O4-->Na2CO3+CO CO3(2-)+2H(+)-->H2O+CO2 (c) 0....

Chem

If you need to prepare 250.0 mL of a pH 5.00 buffer tha t has a total buffer concentration of acetic acid + sodium acetate of 0.050 M, how many moles of each will you need to prepare the solution? Given solutions of acetic acid and sodium acetate with concentrations of 0.10 M ...

chemistry

the formula weight of h2so4 is 98. the weight of the acid in 400ml of 0.1M so the solution is?

Chemisty

Based on the standard analytical practice during a titration of taking pH-volume measurements every 0.3 pH units, how many measurements should you take between the addition of 5mL and 7.5mL of titrant, a) in the titration of a 10mL aliquot of standard 0.1500M TRIS base (Kb=1....

CHEMISTRY

A BUFFER SOLUTION IS MADE FORM 0.10 MOLES NaF AND 0.25 MOLES OF HF IN 1 LITER OF WATER ----WHAT WILL THE BUFFER CAPACITY BE IF 1.0M HCL IS ADDED -----WHAT IF 2.0 M OF NAOH IS ADDED?? PLEASE HELP I HAVE NO CLUE WHERE TO EVEN BEGIN!!! Calculate the pH of the solution using the ...

chemistry

Describe the laboratory preparation of z1Litre of 1.3M formic acid buffer of pH 3.9 from solid sodium formats (molecular weight =68) and formic acid liquid 30% pure (Density of formic acid = 1.029g/ml, pKa = 3.75)

Chemistry

Explain the laboratory preparation of one Litre of 1.3M formic acid buffer of pH 3.9 from solid sodium formate (molecular weight =68) and formic acid liquid 30% pure (Density of formic acid = 1.029g/ml, pKa = 3.75)

chemistry

If you need to prepare 250.0 mL of a pH 5.00 buffer tha t has a total buffer concentration of acetic acid + sodium acetate of 0.050 M, how many moles of each will you need to prepare the solution? Given solutions of acetic acid and sodium acetate with concentrations of 0.10 M ...

CHEMISTRY

Calculate the amount (in mL) of 1.520M NaOH that is required to add the following acetic acid solution to prepare a buffer with the corresponding pH: 30.00mL of a 5.00% (w/v%) acetic acid; the resulting acetate buffer has a pH of 5.75. pKa of acetic acid = 4.74

Chemistry

You are asked to prepare 500. mL of a 0.150 M acetate buffer at pH 5.00 using only pure acetic acid, 3.00 M NaOH, and water. Calculate the quantities needed for each of the following steps in the buffer preparation. 1. Add acetic acid to ~400 mL of water in a 500 mL beaker. ...

chemistry

Choices: True,False. Select all that are True. The pH at the equivalence point of a weak base with a strong acid is expected to be less than 7 because of the presentce of the conjugated acid in the water. One cannot prepare a buffer from a strong acid and its conjugated base, ...

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