If you make up a solution of 100mL of 0.1M HEPES in the basic form, what will be the pH

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Biochemistry

If you make up a solution of 100mL of 0.1 M Hepes in the basic form, what will be the pH? pKa = 7.55

Biochem

HEPES, is a commonly used component of physiological buffers. The PKa of the protonated amino group of HEPES is 7.55 at 25C. Describe the preparation of 1.00L of 0.100M HEPES buffer, pH 8.00, from the sodium salt of the basic form of HEPES (MW 260.3), .0M HCL and deionized water

Biochemistry

Sorry! HEPES, is a commonly used component of physiological buffers. The PKa of the protonated amino group of HEPES is 7.55 at 25C. Describe the preparation of 1.00L of 0.100M HEPES buffer, pH 8.00, from the sodium salt of the basic form of HEPES (MW 260.3), 6.0M HCl and ...

biochemistry

If you make up a solution of 100 mL of 0.1 M HEPES in the basic form, what will be the pH? I have no idea where to even start...

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You have a 0.5 M HEPES pH 7, and a 0.5 M HEPES pH 8 solution. How much of each stock solution do you need to make 125mL of a 50 mM HEPES pH 7.5 solution? How much water?

chemistry

The pKa of HEPES is 7.55 at 20 degrees C, and its MW is 238.31. Calculate the amounts of HEPES in grams and of 1.0 M NaOH in milliliters that would be needed to make 300 mL of 0.2 M HEPES buffer at pH 7.2.

science(chem)

The pKa of HEPES is 7.55 at 20C and it's MW is 238.31. Calculate the amounts of HEPES in grams and of 1.0M NaOH in ml that would be needed to make 300ml of 0.2M HEPES buffer at 7.2. I'm not sure how to go about doing this problem. Help please.

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If you start with 200 ml of 0.1M HEPES buffer (pH 7.5) and add 1.25 ml of 0.5M HCl, what will the final pH be? (The pKa of HEPES is 7.5)

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I have already posted this and never got a response, please help. here is my data from lab: Buffer1: HPO4- weight 3.412g; original pH Buffer 2: HEPES wt: 1.090g original pH 10.08 buffer1: pH of 0.1M: 7.5; pH of 0.01: 7.72; pH of 0.001M: 7.87 buffer2: PH of 0.1M: 7.5 ph of 0.01...

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tough pH problem #2 possible solution

Using the HH equation. pH = pKa + log (base/acid) . I chose pH of 7.45 because I want to minimize the amount of Hepes I have to use so making the pH higher will mean less HEPES would have had to be added originally.. plug in pH of 7.45 and pka of 7.55 and solve for the base/...

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We will need 0.5L extraction buffer with 25 mM HEPES, 1 mM EDTA, and 1 mM DTT. Please calculate how many grams of each solute are needed. (HEPES 238.3; EDTA 292.2; DTT 154.2)

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Here is the hint I received on how to solve those tough pH problems I posted earlier: Both of these can be solved using the H-H and doing the algebra. The math itself is hard to type out, but in Q1 the values of [NaA] and [HA] are 0.04805 and 0.15195. In Q2 you will have to ...

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chemistry

The administration of 100mg or more of morphne (molar mass 283) to a patient would be lethel. which of the following would you consider to be a safe dose. a. 1ml of a 1 percent solution (w/v) b. 1ml of a 0.05 M solution c. 1ml of a 100ml/mg solution d. 0.5ml of a 1M solution e...

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