# How to use serial dilution to dilute a stock solution at 15000 fold dilution?

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### dilution please help

How to use serial dilution to dilute a stock solution at 15000 fold dilution?

### Dilution

Please, if possible, check if my method of dilution is correct Problem: Given a very concentrated liquid PBS, dilute it to 1:15,000 My approach is to use serial dilution First: dilute it 15x (50ul of PBS + 750ul of water)... this will give 1:15 Second: dilute 10xfold more (...

### chemistry

Please help me with this dilution. Given a very concentrated tube of virus. Apply serial dilution to make 1:7500 dilution. Dilute virus in PBS

### Chemistry

1) If I add 10 grams of salt into 0.1 L of water, what is the molarity of the solution? 2) Serial dilution is a common technique to make low concentration solutions. Each Serial Dilution involves taking half the solution and mixing it with a solvent, to halve the concentration...

### Chemistry

A laboratory technician is required to prepare a quarter normal saline solution (0.22% w/v) from the stock normal saline solution. 1. What is the dilution factor required? I have 4-fold dilution - is that correct? 2. Ise this dilution factor to calculate the volume of stock ...

### biology

You have a stock solution that is 350g/L . You perform a 9-fold serial dilution four times. What concentrations will you have in each of your diluted tubes in g/L? (Separate your answers with a comma.)

### Calculating Dilutions

1. 1 ml serum added to 5 ml saline. 1:2 dilution is made. What is the dilution of serum in tube 6? So the serum + saline would make a 1:6 dilution. Multiply that by serial dilution of 1:2 which would give: (1/6) x (1/2) x (1/2) x (1/2) x (1/2) x (1/2) = (1/192). Is that ...

### Clinical Chemistry

I am not sure how to start this. You are given a 200% solution of a compound. You must dilute and construct a calibration curve to interpret concentration of compound in unknown samples. Show the steps needed to perform a two-fold serial dilution through 5 tubes.

### Chemistry

Given a very concentrated glycercol solution. Dilute it to 7.5 fold dilution. How do I do this ?

### CHEMISTRY

How would you prepare 750 ml of a 10 fold dilution of a 17 M solution of acetic acid? How would you prepare 200 ml of a 20 fold dilution of a 17 M solution of acetic acid? please explain and show me the calculations.

### chemistry

What does 1.5 fold dilution and 2.5 fold dilution mean?

### chemistry

What does 1.5 fold dilution and 2.5 fold dilution mean?

### Chemistry (please help)

How would you make a serial dilution if you have 100mM of X These are the values of X that she gave. X 0 1 3 1 10 30 100 These are the values of X in the serial dilution. If you could help me out by start me off because I am kind of confused with the question. Help is ...

### Chemistry

2 ml of stock antibiotic (64 mg/ml) was diluted into a test tube containing 2 ml of broth (final volume is 4). A series of 2-fold SERIAL DILUTIONS were performed by transferring 2 ml to three other tubes, consecutively (final volumes now 2 ml in each tube). The final ...

### Microbiology

2 ml of stock antibiotic (64 mg/ml) was diluted into a test tube containing 2 ml of broth (final volume is 4). A series of 2-fold SERIAL DILUTIONS were performed by transferring 2 ml to three other tubes, consecutively (final volumes now 2 ml in each tube). The final ...

### Science

2 ml of stock antibiotic (64 mg/ml) was diluted into a test tube containing 2 ml of broth (final volume is 4). A series of 2-fold SERIAL DILUTIONS were performed by transferring 2 ml to three other tubes, consecutively (final volumes now 2 ml in each tube). The final ...

### Biology

You begin with 10 ml of 2% acetic acid, and you add 90 ml of water. We will call the resulting solution "dilution#1". What is its concentration? You then repeat the dilution exactly, using 10ml of Dilution #1, adding 90 ml of water. We will call the resulting solution "...

### Chemistry (please help)

How would you make a serial dilution if you have a starting value 100mM of X The are the values of X show different dilutions. I think she wants to know starting from 100mM of X how you would get the X values through a serial dilution. X 0 1 3 1 10 30 100 If you could help me ...

### Chemistry

A: A student must make a five-fold dilution of a 2.15 M HCl solution. If the student wishes to make 75.0 mL of the diluted solution, what volume of the 2.15 M solution will he need to dilute? B: What is the concentration of the diluted substance?

### Biology

You have a stock solution of 100mg/100ml methylene blue and you need 6.0 ml of final solution at 2mg/100ml. Design a serial dilution using volumes between 0.1 and 10 ml. Final volume must be 6ml.

### chemistry (please check/help)

How would you make a serial dilution if you have a starting value 100mM of X The are the values of X show different dilutions. Volume variables (x) mL 0 1 3 1 10 30 100 We starting out with 100mM of X which would be considered its concentration. In order to describe how to do ...

### chemistry (work shown)

How would you make a serial dilution if you have a starting value 100mM of X The are the values of X show different dilutions. Volume variables (x) mL 0 1 3 1 10 30 100 We starting out with 100mM of X which would be considered its concentration. In order to describe how to do ...

### Chemistry

Prepare a 0.0200 mM dye solution at low pH by dilution 0.40 ml of the stock 0.100 mM chemical X into the appropriate volume of 2.5 M HCl. What is the amount of HCL required for dilution, the final concentration of HCl in the solution, and the pH? I know there's the MV = MV, ...

### chemistry

What is a serial dilution and why might you want to use it?

### science

You need to make a 2 fold serial dilution starting at 1:100 and ending at 1:3200. You need 2 ml in each test tube when you are finished. You have 40 microliters of serum. do you have enough?

### Chemistry (URGENT)

Calculate the molarity of the Co^2+ ion in each of the solutions 1-5. The new molarity is given by the molarity of the stock solution multiplied by the dilution factor. Solution: Co(NO3)2 Original molarity: 0.160 Dilution #1: none D#2: 12mL to 16mL D#3: 8mL to 16mL D#4: 4mL to...

### college

A solution was diluted by a factor of 2 and then again using a 1 to 3 dilution. What is the dilution of concentrate in the final sample

### Chemistry

You have a bottle of 20% NaOH. You will use some of it to make a new solution by dilution with water. How musch 20% NaOH is needed to prepare 150mL of 7% NaOH by dilution.

### chemistry

1. calculate the volume of concentrated HNO_3 (68%)that is needed to prepare stock solution 0.25M in 1000mL volumetric flask. (density HNO_3 = 1.513 g/cm^3) 2. from above stock solution, determine volume needed to dilute to 500mL volumetric flask that contained 10 times dilution.

### Chemistry

1. calculate the volume of concentrated HNO3 (68%)that is needed to prepare stock solution 0.25M in 1000mL volumetric flask. (density HNO3 = 1.513 g/cm^3) 2. from above stock solution, determine volume needed to dilute to 500mL volumetric flask that contained 10 times dilution.

### chemistry, biotechnology

The question is: "The molar extinction coefficient of pure antibody is 1.4 absorbance units per milligram antibody @280nm. You have a concentrated antibody solution, so you make a 5-fold dilution and measure @280, getting an absorbance of 0.845. What is the concentration of ...

### Chemistry (Dilution) Help please?

Calculate how to prepare 100.0mL of a 1.25x10^-4 M aqueous solution of Fe(NO3)3(aq) by dilution of stock of 0.0025M Fe(NO3)3(aq) in a 100.0mL volumetric flask? I tried using the m1v1=m2v2 equation but it doesn't look like that equation would work for this problem??

### science(chem)

I'm making different concentrations of ethanol and I just wanted to check my calculations and method. Making ethanol concentrations of: 0.40% 0.30% 0.20% 0.10% 0.05% To make the 0.40% ethanol from stock solution using 100ml volumetric flask: (xg ethanol)/(100ml) x 100= 0.40% ...

### biology

You carried out a 10-fold dilution series. You started with tube 0 which contained an overnight culture of E. coli. You estimated the original culture contained 6 x 108 cell/ml using McFarland nephlemeter standards. You plated 0.1 ml volumes of a number of the dilutions. Which...

### chemistry

A student needs to make a dilution from a stock solution. The stock solution is 2.) M NaCl. How much should the student use of the 2.0 M NaCl to make 1.0 L of 0.05M NaCl diluted stock.

### chemistry

if i have a 21% solution of a product, and i need to make it a 9% solution of the same product what do i do? i am trying to dilute a product that is 21% to a 9% soultion so it can safety be used on cats. i know what to dilute it with, but i do not know how much or the math to ...

### Chemistry

A stock aqueous solution contains 348.29 grams of NaCl per 1.000L. 68.00mLs of water is added to 10.00mLs of the stock solution. What is the molarity of the dilution???

### biotechnology

i want secondary antibody dilution in 1:15000 in PBS. How i should do it

### Chemistry

If 455-ml of 6.0 M HNO3 is used to make a 2.5 L dilution,what is the molarity of the dilution?

### Biochemistry

Preform dilution calculations for 10 ml of 2 M stock solution. Molecular weight for the component is 48.6 g / mol

### Chemistry

describe the preparation of 12 mL of a 0.050 M solution of each of the halogens, using the reactions in the preceding question. Available to you are solid Ca(OCl)2; 0.20 M solutions of NaCl, NaBr, NaBrO3, and CuSO4; a 0.40 M solution of NaI; a 0.50 M solution of H2SO4; ...

### Science

You have a stock solution of 3.5M glucose. You need a working solution of 0.5M glucose in a volume of 400ml. Explain how you make the solution. Also what is the concentration of the dilution solution?

### Chemistry

What is the molarity of the final solution if 2.00mL of the 5.00 M stock solution is diluted to 10.00mL to make solution A? Solution A then undergoes a second dilution by taking 3.00mL solution A and diluting it to 25mL.

### Chemistry

What is the molarity of the final solution of 2.00mL of the 5.00M stock solution is diluted to 10.00mL to make solution A. Solution A undergoes a second dilution by taking 3.00mL solution A and diluting it to 25mL? I'm so confused by this. Please help.

### chemistry

10.0mL of solution containing 20.0 mL of 1.0 M NaX (unknown base) and 10.0 mL of 1.0 M HX (unknown acid) was diluted with 50.0 ml water. The pH is 4.92. What is the concentration of HX after dilution and X- after dilution? What is the equilibrium concentration of HX, X-, and ...

### chemistry

Hey, I am having a real hard time getting the answers to the problems. Any help would be greatly aprreciated! 1. When 11 mL of a solution containing 2.9 M AlBr3 is diluted to 33 mL, a dilute solution is formed. What is the Br- concentration in 25 mL of the dilute solution? 2. ...

### Chemistry

After the initial 1 mL of 0.2 M FeCl3 (aq) and 1 mL of 0.2 M KSCN (aq) were combined it was then diluted with an additional 130 mL of deionized water. Which way would this dilution cause the equilibrium to shift? Is a dilution going to be a stress in all situations?

### science

3ml of 2M NaOH is added to 27 ml of destilled water. what dilution is made? what is the concetration of Naoh in the dilution?

### chem

A student based the analysis of Cu(II) in a sample on the formation of the blue complex [Cu(H2O)6]+2. A standard solution was made when 1.276g of pure copper wire (MM= 63.546g/mol) was weighed, dissolved in concentrated HNO3, and diluted to 1.00x102mL. The stock solution was ...

### Chemistry

I've been given the Volume of Concentrated NaOh solution (mL) and also the Concentration of Concentrated NaOh solution (M). I also have the volume of stock solution after dilution (mL). The question is asking the approximate concentration of stock solution (M) any help?

### chemistry dilution

Given VLP with concentration 200ug/mL, dilute to 3ug/100ul. Please help me with this; many thanks

### chemistry (continued)

i kinda understand waht you're saying. to be a bit more clear this is from a lab, and here are the volumes we retrieved: Volume of Concentrated NaOH soultion (mL) = 9.00 mL Concentration of Concentrated NaOH soultuon (M) = 6.00 M Volume of Stock Solution after Dilution (mL) = ...

### chemistry

I have 3 grams of CaCI2 and dissolve this into 0.5 L water. What is the concentration in g/dl (grams/deciliter)? I take 10 milliliters of this solution and add it to 990 milliliters of watet fo a total of 1000 milliliters. What is the concentration of this new dilution in ...

### Chemistry

You have a stock solution of 14.4 M NH3. how many mililiters of this solution should you dilute to make 1050mL of .220 M NH3 answer 16.o mL Question if you take a 10.5mL portion of the stock solution and dilute it to a total volume of .600L, what will be the concentration of ...

### Science

Take 1mL of 1M NaCl solution, and add 9mL of water. Then take 10microL of the diluted solution and add 40microL of water. What is the dilution? And what is the final concentration of NaCl? So I see how this is a 2:1 dilution, but I am having a hard time figuring out the final ...

### science

Take 1mL of 1M NaCl solution, and add 9mL of water. Then take 10microL of the diluted solution and add 40microL of water. What is the dilution? And what is the final concentration of NaCl? So I see how this is a 2:1 dilution, but I am having a hard time figuring out the final ...

### Chemistry 2

You have a stock solution of 15.0 M NH3 . How many milliliters of this solution should you dilute to make 1200ml of 0.280 M NH3? If you take a 15.0ml- portion of the stock solution and dilute it to a total volume of 0.600 L , what will be the concentration of the final ...

### chemistry

In performing an immunological procedure, you add 2 mL of serum to 8 mL of saline. Two 1:2 serial dilutions are then made. What is the dilution of serum contained in the final tube?

### chemistry

e) In performing an immunological procedure, you add 2 mL of serum to 8 mL of saline. Two 1:2 serial dilutions are then made. What is the dilution of serum contained in the final tube?

### chem

quantitative determination of acetylsalicylic acid in aspirin tablets by back titration 1)why do we use a more dilute NaOH solution for the standardization of NaOH? 2)what is the rationale behind the dilution and aliquoting of the aspirin sample? 3)what are the principles ...

### chemistry - dilution factor

I have 4 ml of eluent from SPE and I brought the volume up to 5 ml using the solvent. The eluent was ran in UV for Quant analysis. Is the dilution factor 0.8 because it would be "4 in 5"?

### Chemistry

A laboratory stockroom has a stock solution of 90%(m/v) sulfuric acid,H2SO4(aq). If a technician dilutes 50ml of the stock solution to a final volume of 300ml, what will be the new mass/volume percent concentration?(HINT: the dilution formula can be used for concentration ...

### gen chem 2

In the chemical kinetics experiment, determine the dilution of the stock bleach solution. The bleach used will be around 5.5% (weight/volume). Convert this to molarity

### Biology

If I have 2.5 amylase stock solution, 2.5 PBS Buffer, and final amylase concentration of 25 [ug/ml] , how do you calculate the amylase dilution factor?

### Lab Math-Dilutions

You have 1.50mL of a urine specimen. The assay you are about to perform requires a 1/25 dilution of this specimen. How much water must be added to the specimen to achieve this dilution? I know this is simple but I am not coming up with the right answer. Thank you to whoever ...

### Chemistry Lab

You are given 350 mL a stock solution of 1.66E-02 M benzoic acid, MW = 122.125 g/mol. Answer the following questions. A. What is the number of moles present in the original stock solution? B. You need to dilute the benzoic acid stock solution to 1.24E-03 M. If you need 455 mL ...

### Chemistry Lab

You are given 350 mL a stock solution of 1.66E-02 M benzoic acid, MW = 122.125 g/mol. Answer the following questions. A. What is the number of moles present in the original stock solution? B. You need to dilute the benzoic acid stock solution to 1.24E-03 M. If you need 455 mL ...

### chemistry

Care must be taken when diluting sulfuric acid with water, because the dilution process is highly exothermic: H2SO4(l) "arrow" H2SO4(aq) + heat a) Find the ÄHo for diluting 1.00 mol of H2SO4(l) (d = 1.83 g/mL) to 1 L of 1.00 M H2SO4(aq) (d = 1.060 g/mL). Suppose you carry out...

### Chemistry

Care must be taken when diluting sulfuric acid with water, because the dilution process is highly exothermic: H2SO4(l) "arrow" H2SO4(aq) + heat a) Find the ÄHo for diluting 1.00 mol of H2SO4(l) (d = 1.83 g/mL) to 1 L of 1.00 M H2SO4(aq) (d = 1.060 g/mL). Suppose you carry out...

### chemistry

During lab, you are provided with a stock antibody solution (Ab) that has a concentration of 400 μg/μl. For lab, it is necessary make the following dilutions (Fill in the blanks): 5μL of Stock Ab + 195μL of buffer to make a 1:____ dilution at _______μg...

### Biology

Grant’s DNA sample has a concentration of 45µg/mL, and he has 350µL of solution. Which of the following answers would allow him to run the proper amount of DNA on a quality control gel? A) He should dilute the sample 1:10 and load 10uL on the gel B) He should complete 2 1:...

### Chem

During your lab , you are provided with a stock antibody solution (Ab) that has a concentration of 400 ug/ul . you are provided with 10 ul( amount of stock antibody ) and 90 ul( amount of buffer ) . find the final dilution factor and the final Ab concentration.

### CHEMISTRY

A STUDENT STUDYING THE FE+3- HSCN equilibrium put into a test tube 10.00ml of 2.00 x 10-3 M Fe(NO3)3 with 10.0 mL of 2.00 x 10-3 M HSCN.THE H+ in the resulting solution was manteined at 0.500.By spectrophotometric analysis of the equilibrium solution, the equilibrium ...

### Dilution

What volume of H2O should be added to 500 ML of a 1.0 mol/L CuSO4 solution to dilute it to 0.5 mol/L? This is what I have so far... H20 + CuSO4 --------> H2SO4 + CuO So you use the formula M1V1 = M2V2 so its (0.500)(1.0) = (0.5)(V) Solve for V, I got 1.0 L. HOWEVER, the ...

### Chemistry

If you dilute 14.0mL of the stock solution to a final volume of 0.350L , what will be the concentration of the diluted solution? Before it, there was a question: How many milliliters of a stock solution of 5.60 M HNO3 would you have to use to prepare 0.190L of 0.450 M HNO3? ...

### chemistry

A STUDENT STUDYING THE FE+3- HSCN equilibrium put into a test tube 10.00ml of 2.00 x 10-3 M Fe(NO3)3 with 10.0 mL of 2.00 x 10-3 M HSCN.THE H+ in the resulting solution was manteined at 0.500.By spectrophotometric analysis of the equilibrium solution, the equilibrium ...

### Science

A liquid solution of formaldehyde is 37% pure. This solution has a specific gravity of 1.037. The molecular weight of formaldehyde is 30.0. To 1.00 ml of this impure stock solution is added quantity sufficient total the total volume to 500 ml. If 1.00 ml of this solution is ...

### Science

We conducted an expirement to detect disulphide groups in protein. One tube(lysozyme) contained unkowns, had approx 9.34 micromoles of disulphide groups. The question asks me to work backwards through all the dilution factors taken to give number of micromoles of disulphide ...

### Chemistry

We conducted an expirement to detect disulphide groups in protein. One tube(lysozyme) contained unkowns, had approx 9.34 micromoles of disulphide groups. The question asks me to work backwards through all the dilution factors taken to give number of micromoles of disulphide ...

### Chemistry

In this experiment absorption spectroscopy is conducted to detect Cr(VI)in aqueous solutions and with it the concentration of Cr(VI). How would each of the following affect the Cr(VI) concentration determined in this experiment? Explain the answer. 1. A student adds more ...

### Organic Chemistry

How am I supposed to do these questions? I'm completely lost. A 0.120 M solution of an enantiomerically pure chiral compound D has an observed rotation of 0.12° in a 1-dm sample container. The molar mass of the compound is 130.0 g/mol. (b) What is the observed rotation if ...

### chemistry

HCL acid is available as a stock solution with a concentration of 10 mol/L. If you need (9.0*10^-1) L of (2.50*10^0) mol/L hydrochloric acid, approximately how much distilled water will you need to make the dilution?

### chemistry

HCL acid is available as a stock solution with a concentration of 10 mol/L. If you need (2.100*10^0) L of (2.3*10^0) mol/L hydrochloric acid, approximately how much distilled water will you need to make the dilution?

### chemistry

Hi, I've watched a tutor solve a simple dilution solutions problem on video. There's a calculation in that problem I wish you could clarify for me. It goes like this: Approximately what volume of water is needed to dilute 25.0 mL of 18.0 M sulfuric acid to 3.0 M ? M * L = M * ...

### science

dilution of a vinegar solution 10x?

### chemistry

What is the concentration of a 1:2 dilution of a 0.6 M NaHCO3 solution?

### Chemistry

Can u tell me the practical details of how you would prepare 0.5mol/dm3 hydrochloric acid solution from a stock of 34% (w/w) with a relative density of 1.18. Figure the number of moles per liter of the concentrate. Say is is 17 for example sake. You want a dilution of 17/.5 or...

### bobpursley

I don't really understand what you mean by "factors"... factors of 2 and factors of 3? If you have a 1.2M concentration, and you want to dilute it to .45M concentration, you are diluting it by a factor of 1.2/.45 or 2.666666. To do that, add 1.66666 parts water, one part ...

### chemistry

what volume of a 0.34M MgCI2, solution is required to make 250ml of a 0.10M solution by dilution?

### chemistry

What volume of a 0.34M MgCL2 solution is required to make 250ml of a 0.10M solution by dilution?

### Chemisty

A 5M solution is diluted 1/10 and again 1/5. What is the concentration of each dilution?

### Chemistry

how would a dilution be performed, starting with a concentration .50 M Cacl2 solution, to obtain 250ml of a .060M solution?

### Chemistry

how would a dilution be performed, starting with a concentration .50 M Cacl2 solution, to obtain 250ml of a .060M solution?

### Determination of ASA Content of Tablets Lab Report

Not asking for answers necesarily but I would to know how to approach each question Mass of pure acetylsalicylic acid (ASA) : .508 g Dilution Absorbance (a.u.) 2:11 .116 4:11 .259 6:11 .392 8:11 .502 10:11 .548 Mass of tablet : .380 g Absorbance read on the Spec 20 : .314 a.u...

### science

how can i prepare this solution? Dissolve 24.82g of sodium thiosulphate in each cubic decimeter of solution to make astock of 0.1M. Dilute this solution 20-fold. URGENT PLEASE!!!!!!!!!!!!!!!

### Biochemistry

I have a 1/40 dilution as 19.18 mg/ml. how would i get back to the original solution?

### chemistry

A solution is made by dilution 400.mL of 2.0 M HCL to a final volume of 1000. mL. what is the pH of this solution? i'm stuck on this question i have no idea what to do, can anyone help? thanks:)

### Chemistry

To what volume must 1580mL of 0.2005 N solution be diluted so that the resulting solution will be 0.1899 M? How much water will be needed in the dilution?

### Chemistry

How would each of the following affect the Cr(VI) concentration determined in this experiment? Justify the answers with an explanation. In the experiment, absorption spectroscopy was used to obtain the concentration of Cr(VI) ions. 1) A student adds more standard solution than...