BSA stock solution A has a concentration of 3.50 mg/mL.
1.00mL of stock solution A is diluted to a final volume of 5.00mL to prepare solution B.
1.00mL of stock solution B is diluted to a final volume of 5.00mL to prepare solution C.
1.00mL of stock solution C is diluted to a final volume of 10.00mL to prepare solution D.
Calculate the concentrations of solutions B, C, and D.
A you are diluting it five times, meaning one part stock, four parts water. Five times means new B concentration is 3.50/5 mg/ml
diluting again five times. concentration of C is 3.5/25 mg/ml
dilting ten times. concentration D is 3.5/250 mg/ml
To calculate the concentrations of solutions B, C, and D, we need to consider the dilution process.
Solution B:
Solution B is prepared by diluting 1.00 mL of stock solution A to a final volume of 5.00 mL. This means the final volume of B is 5.00 mL.
To calculate the concentration of solution B, we can use the formula:
Concentration B = (Volume A * Concentration A) / Volume B
Plug in the values:
Concentration B = (1.00 mL * 3.50 mg/mL) / 5.00 mL
Simplify:
Concentration B = 0.70 mg/mL
So, the concentration of solution B is 0.70 mg/mL.
Solution C:
Solution C is prepared by diluting 1.00 mL of solution B to a final volume of 5.00 mL.
Using the same formula as before, we have:
Concentration C = (Volume B * Concentration B) / Volume C
Plug in the values:
Concentration C = (1.00 mL * 0.70 mg/mL) / 5.00 mL
Simplify:
Concentration C = 0.14 mg/mL
So, the concentration of solution C is 0.14 mg/mL.
Solution D:
Solution D is prepared by diluting 1.00 mL of solution C to a final volume of 10.00 mL.
Using the formula again, we have:
Concentration D = (Volume C * Concentration C) / Volume D
Plug in the values:
Concentration D = (1.00 mL * 0.14 mg/mL) / 10.00 mL
Simplify:
Concentration D = 0.014 mg/mL
So, the concentration of solution D is 0.014 mg/mL.