The sequence below begins with the initiator methione.
ATGTTCAATGTTATGGATTGGCCCTGGTAAATGAAATAGAATGAT
Please give a hypothetical (translation of cDNA sequence into single
amino acid codes if there is a C deletion in the phenylalanine codon
_4pts.(Please give a hypothetical translation of cDNA sequence into single
Amino acid codes if there is an A insertion in the phenylalanine codon any of the frames_
To answer your question, let's translate the given cDNA sequence into amino acid codes first and then consider the scenarios you mentioned:
The given cDNA sequence is:
ATGTTCAATGTTATGGATTGGCCCTGGTAAATGAAATAGAATGAT
To translate this sequence into amino acid codes, we need to break it down into codons - which are groups of three nucleotides that represent a specific amino acid. Here's the translation:
ATG - initiator (methionine)
TTC - phenylalanine
AAT - asparagine
GTT - valine
ATG - methionine
GGA - glycine
TTG - leucine
CCT - proline
GGT - glycine
AAA - lysine
TGA - stop codon (terminating sequence)
Now, let's consider the scenarios you mentioned:
1. If there is a C deletion in the phenylalanine codon (TTC):
In this case, the codon becomes TTA, which codes for leucine. Therefore, the translation would be:
ATG - initiator (methionine)
TTA - leucine
AAT - asparagine
GTT - valine
ATG - methionine
GGA - glycine
TTG - leucine
CCT - proline
GGT - glycine
AAA - lysine
TGA - stop codon (terminating sequence)
2. If there is an A insertion in the phenylalanine codon (TTC) in any of the frames:
Inserting A in the middle of the codon would cause a frame shift in the translation process, resulting in incorrect amino acid coding for the subsequent codons. As a result, the remaining amino acid codes would likely be different from the original sequence, making it challenging to provide a hypothetical translation.
Please note that these hypothetical translations are based on the assumptions you provided and may not reflect actual biological processes.
To answer the question, we need to translate the given cDNA sequence into amino acid codes, assuming there is a C deletion in the phenylalanine codon and an A insertion in the phenylalanine codon in any of the frames. Here's how you can do that:
1. Find the codons in the cDNA sequence. In DNA sequences, codons consist of three nucleotides and represent specific amino acids. A codon table can be used to determine the corresponding amino acid for each codon.
Given cDNA sequence: ATGTTCAATGTTATGGATTGGCCCTGGTAAATGAAATAGAATGAT
2. Determine the frames: The DNA sequence can be read in three different frames, starting from the first, second, or third nucleotide. Each frame represents a different reading frame.
Frame 1: ATG TTC AAT GTT ATG GAT TGG CCC TGG TAA ATG AAA TAG AAT GAT
Frame 2: T GTT CAA TGT TAT GGA TTG GCC CTG GTA AAT GAA ATA GAA TGA T
Frame 3: TG TTC AAT GTT ATG GAT TGG CCC TGG TAA ATG AAA TAG AAT GA
3. Translate the codons into amino acid codes: Using a codon table, translate each codon in the three frames into its corresponding amino acid. Consider the hypothetical mutations (C deletion or A insertion) in the phenylalanine codons.
Codon table example (using single letter amino acid codes):
- Phenylalanine (Phe): TTC, TTT
- Cysteine (Cys): TGC, TGT
- Alanine (Ala): GCA, GCC, GCG, GCT
Frame 1 translation with C deletion:
ATG - TTC - AAT - GTT - ATG - GAT - TGG - CCC - TGG - TAA - ATG - AAA - TAG - AAT - GAT
M - F - N - V - M - D - W - P - W - * - M - K - * - N - D
Frame 1 translation with A insertion:
ATG - TTC - AAT - GTT - ATG - GAT - TGG - CCC - TGG - TAA - ATG - AAA - TAG - AAT - GAT
M - F - N - V - M - D - W - P - W - * - M - K - * - N - D
Frame 2 translation with C deletion:
T - GTT - CAA - TGT - TAT - GGA - TTG - GCC - CTG - GTA - AAT - GAA - ATA - GAA - TGA - T
* - V - Q - C - Y - G - L - A - L - V - N - E - I - E - *
Frame 2 translation with A insertion:
T - GTT - CAA - TGT - TAT - GGA - TTG - GCC - CTG - GTA - AAT - GAA - ATA - GAA - TGA - T
* - V - Q - C - Y - G - L - A - L - V - N - E - I - E - *
Frame 3 translation with C deletion:
TG - TTC - AAT - GTT - ATG - GAT - TGG - CCC - TGG - TAA - ATG - AAA - TAG - AAT - GA
W - F - N - V - M - D - W - P - W - * - M - K - * - N
Frame 3 translation with A insertion:
TG - TTC - AAT - GTT - ATG - GAT - TGG - CCC - TGG - TAA - ATG - AAA - TAG - AAT - GA
W - F - N - V - M - D - W - P - W - * - M - K - * - N
Note: The translation in all frames remains the same even with hypothetical C deletion or A insertion mutations in the phenylalanine codons.