Consider a solution containing 4.35 mM of an analyte, X, and 1.09 mM of a standard, S. Upon chromatographic separation of the solution peak areas for X and S are 3251 and 10075, respectively. Determine the response factor for X relative to S.

F=?

To determine the concentration of X in an unknown solution, 1.00 mL of 8.01 mM S was added to 4.00 mL of the unknown X solution and the mixture was diluted to 10.0 mL. After chromatographic separation, this solution gave peak areas of 5969 and 4435 for X and S, respectively. Determine the concentration of S in the 10.0 mL solution.
S=?mM

Determine the concentration of X in the 10.0 mL solution.
X=?mM

Determine the concentration of X in the unknown solution.
X=?mM

To determine the response factor for X relative to S, we can use the equation:

Response Factor (F) = (Peak Area of X) / (Peak Area of S)

In this case, the peak areas for X and S in the first solution are given as 3251 and 10075, respectively. Therefore, the response factor is:

F = 3251 / 10075 = 0.3221 (rounded to four decimal places)

Now, let's move on to the second question.

To determine the concentration of S in the 10.0 mL solution, we can use the equation:

Concentration of S = (Peak Area of S / Response Factor) / (Volume of the Solution)

In this case, the peak area of S is given as 4435. Given that the response factor (F) is 0.3221 and the volume of the solution is 10.0 mL, we can calculate:

Concentration of S = (4435 / 0.3221) / 10 = 137.6430 (rounded to four decimal places)

Therefore, the concentration of S in the 10.0 mL solution is 137.6430 mM.

Moving on to the next question.

To determine the concentration of X in the 10.0 mL solution, we can use a similar equation:

Concentration of X = (Peak Area of X / Response Factor) / (Volume of the Solution)

In this case, the peak area of X is given as 5969. Using the same response factor (F) of 0.3221 and the volume of the solution as 10.0 mL, we can calculate:

Concentration of X = (5969 / 0.3221) / 10 = 185.1993 (rounded to four decimal places)

Therefore, the concentration of X in the 10.0 mL solution is 185.1993 mM.

Finally, let's determine the concentration of X in the unknown solution.

To do this, we can use the equation:

Concentration of X (in unknown solution) = [Concentration of X (in 10.0 mL solution) * (Volume of the unknown solution / Volume of the 10.0 mL solution)]

In this case, we're given that the volume of the unknown solution is 4.00 mL. Plugging in the values, we can calculate:

Concentration of X (in unknown solution) = [185.1993 * (4.00 / 10.0)] = 74.0797 (rounded to four decimal places)

Therefore, the concentration of X in the unknown solution is 74.0797 mM.