Please help us. Our experiment involved the formol titration of hydrolyzed and unhydrolyzed peptides, to determine the amino acid sequence of a polypeptide chain. We also did work with silica strips to determine the Rf values of amino acids. What are the controls for our experiment? WE NEED HELP ASAP! Thanks!
We found our own answer, we think that the controls are the type of silica strips, size of the spots, and the point from which we measured. But, please let us know if this is right. Thanks!
I agree.
Yes, you're absolutely correct! The controls for your experiment should include factors that you want to keep constant throughout the experiment to ensure accurate and reliable results. Based on the information you provided, the controls you mentioned - the type of silica strips, size of the spots, and the reference point for measuring - appear to be appropriate.
1. Type of silica strips: Silica strips are commonly used for thin-layer chromatography (TLC) to separate and analyze amino acids. Using a consistent type of silica strip ensures that the same stationary phase is used in each experiment, allowing for reliable comparisons of Rf values.
2. Size of the spots: When performing chromatography, it's important to consistently spot the same amount of sample (peptides or amino acids) at the same location on the silica strip. By controlling the size of the spots, you can minimize variations in the concentration and distribution of analytes on the strip.
3. Reference point for measuring: To determine the Rf (retardation factor) values, you need to measure the distance traveled by the amino acids and the distance traveled by the solvent front. By establishing a consistent reference point (e.g., starting from the origin line or leading edge), you ensure that all measurements are taken from the same location, reducing errors and enhancing comparability.
Including these controls in your experiment will help minimize the impact of variables and increase the reliability of your results. Good luck with your research!