This is the homework exercise:

You want to add 0.13 ul of DNA to 4.87 ul of extraction mix to have a final total of 5.0 ul of sample. But you don't want to make 0.13 aliquots because it is too small. Make a dilution to accomodate for the small aliquot.

Any help would be great thanks

To make a dilution to accommodate for the small aliquot, you can follow these steps:

1. Calculate the dilution factor:
- Divide the desired final volume by the volume of the small aliquot.
- In this case, the desired final volume is 5.0 ul, and the volume of the small aliquot is 0.13 ul.
- So, the dilution factor is 5.0 ul / 0.13 ul = 38.46 (rounded to two decimal places).

2. Prepare a dilution by mixing the small aliquot with an appropriate amount of diluent. This will allow you to achieve the desired final volume while accommodating the small aliquot.

- To calculate the volume of diluent needed:
- Multiply the volume of the small aliquot by the dilution factor.
- Subtract the volume of the small aliquot from the result to get the volume of diluent.
- In this case, the volume of the small aliquot is 0.13 ul, and the dilution factor is 38.46.
- So, the volume of diluent needed is (0.13 ul) * (38.46) - (0.13 ul) = 4.87 ul.

3. Mix the small aliquot with the calculated volume of diluent.
- In this case, you would mix 0.13 ul of DNA with 4.87 ul of diluent to achieve a final volume of 5.0 ul.

By making this dilution, you can accommodate the small aliquot of 0.13 ul into a larger volume of 5.0 ul without having to work with such a small volume directly.