Devise a plan to carry out the following growth curve:

Seed cells on day -1.

Transfer cells to the test media on day 0.

From day 0, plan to carry out your growth curve for 10 days.

During this time, the cells are to be fed three times (excluding the day 0 medium change), and enumerated 4 times.

The test media conditions are medium that contains 4% serum and medium that contains 12% serum.

How many culture vessels do you need?

How much media do you need? This should include all procedures.

When are you going to do what?

To devise a plan to carry out the growth curve as described, we need to break down the steps and calculate the resources required.

Step 1: Seed cells on day -1.
- This step requires preparing the cell suspension and inoculating the cells into culture vessels. The number of culture vessels needed will depend on the desired cell density and the type of vessels being used. Let's assume we want to seed the cells at a density of 1 million cells/mL, and we will use 10 mL culture vessels. Therefore, we will need 10 million cells and 10 culture vessels.

Step 2: Transfer cells to the test media on day 0.
- This step involves transferring the seeded cells from the initial culture vessels to new vessels containing the test media. Since we started with 10 culture vessels, we will transfer the cells to 10 new vessels containing the appropriate media conditions (4% serum and 12% serum).

Step 3: Feeding the cells three times during the 10-day growth curve.
- To feed the cells three times, we will need to refill the culture vessels with fresh media. Assuming we discard the old media and fully replace it each time, we will need enough media to fill the culture vessels 3 times. If each culture vessel has a volume of 10 mL, we will need a total of 10 mL x 3 = 30 mL of media for feeding.

Step 4: Enumerating the cells four times during the 10-day growth curve.
- Enumerating the cells refers to counting the number of cells present at different time points. This requires removing a small sample from each culture vessel and performing cell counting using a suitable method. Let's assume we will use a standard hemocytometer for cell counting. Each counting requires a small volume of sample. If we assume we will use 1 mL of sample for each enumeration, then we will need 1 mL x 4 = 4 mL of media for enumeration.

Summary of media requirements:
- Seeding cells: 10 mL (initial culture vessels)
- Feeding: 30 mL
- Enumeration: 4 mL

Summary of culture vessels required:
- Seeding cells: 10 culture vessels
- Transfer to test media: 10 culture vessels

Timing:
- Day -1: Seed cells
- Day 0: Transfer cells to test media
- Day 1-10: Feed the cells on days 1, 5, and 9
- Day 1-10: Enumerate the cells on days 1, 4, 7, and 10

Note: It is important to adjust the actual volumes and timings based on the specific cell culture protocols, growth rates, and requirements of the experiment at hand.