time day 0 day 1 day 3 day 4 day 5 day 7
count
blank 7 10 5 14 9 8
low 45&49 64&70 62&69 75&79 80&82 68&72
high 45&49 72&78 100&112 180&195 250&267 208&226
Calculate the cell yield for the following data that were obtained using a coulter counter. Conditions:
machine counting volume: 0.5 mL
PBS in Vial: 15.0 mL
Sample added: 0.2 mL
Total sample size:
Day 0: 1.0 mL
Day 1: 1.0 mL
Day 3: 2.0 mL
Day 4: 2.0 mL
Day 5: 2.5 mL
Day 7: 3.0 mL
To calculate the cell yield for each day, we need to use the formula:
Cell yield = (count / machine counting volume) x (Total sample size / Sample added)
Let's go through each day's calculation:
Day 0:
- Count: 7
- Machine counting volume: 0.5 mL
- Total sample size: 1.0 mL
- Sample added: 0.2 mL
Cell yield = (7 / 0.5) x (1.0 / 0.2)
= 14 x 5
= 70
Day 1:
- Count: 10
- Machine counting volume: 0.5 mL
- Total sample size: 1.0 mL
- Sample added: 0.2 mL
Cell yield = (10 / 0.5) x (1.0 / 0.2)
= 20 x 5
= 100
Day 3:
- Count: 5
- Machine counting volume: 0.5 mL
- Total sample size: 2.0 mL
- Sample added: 0.2 mL
Cell yield = (5 / 0.5) x (2.0 / 0.2)
= 10 x 10
= 100
Day 4:
- Count: 14
- Machine counting volume: 0.5 mL
- Total sample size: 2.0 mL
- Sample added: 0.2 mL
Cell yield = (14 / 0.5) x (2.0 / 0.2)
= 28 x 10
= 280
Day 5:
- Count: 9
- Machine counting volume: 0.5 mL
- Total sample size: 2.5 mL
- Sample added: 0.2 mL
Cell yield = (9 / 0.5) x (2.5 / 0.2)
= 18 x 12.5
= 225
Day 7:
- Count: 8
- Machine counting volume: 0.5 mL
- Total sample size: 3.0 mL
- Sample added: 0.2 mL
Cell yield = (8 / 0.5) x (3.0 / 0.2)
= 16 x 15
= 240
So, the cell yield for each day is as follows:
Day 0: 70
Day 1: 100
Day 3: 100
Day 4: 280
Day 5: 225
Day 7: 240