Please help me with this dilution.
Given a very concentrated tube of virus. Apply serial dilution to make 1:7500 dilution. Dilute virus in PBS
To make a 1:7500 dilution of the concentrated virus in PBS, you can follow these steps:
1. Start by labeling your tubes or containers appropriately. You will need two tubes for this dilution.
2. Take 1 mL of the concentrated virus and add it to the first tube labeled "Tube A." This is your stock solution.
3. Take 1 mL of PBS (phosphate-buffered saline) and add it to the second tube labeled "Tube B." This is your diluent.
4. To create the 1:7500 dilution, you need to transfer a specific volume of the stock solution into the diluent. The dilution factor represents the ratio of the final volume to the initial volume. In this case, the dilution factor is 1:7500.
5. To calculate the volume of stock solution required, divide the volume of the final solution by the dilution factor:
Volume of stock solution = Volume of final solution / Dilution factor
Volume of stock solution = 1 mL / 7500
Volume of stock solution = 0.0001333 mL (rounded to four decimal places)
6. Pipette 0.0001333 mL (or the nearest possible volume) of the stock solution from Tube A and add it to Tube B containing the PBS diluent. Mix well by gentle swirling or pipetting up and down.
7. Tube B now contains a 1:7500 dilution of the concentrated virus. To ensure thorough mixing, you can perform additional serial dilutions by transferring a specific volume from Tube B to fresh tubes containing the diluent.
That's it! You have successfully performed a 1:7500 dilution of the concentrated virus using the serial dilution method with PBS as the diluent.